LOCUS       sequence-276450-         807 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector, with insert of the envelope (E) 
            gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554."
COMMENT     Sequence Label: pDONR207 SARS-CoV-2 E
FEATURES             Location/Qualifiers
     source          1..807
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    60..84
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(313..412)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(524..543)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(602..621)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 tttgantgat agtgacctgt tcgttgcaac aaattgatga gcaatgcttt tttataatgc
       61 caactttgta caaaaaagtt ggcatgtact ctttcgtgag cgaggaaacc ggcaccctga
      121 tcgtgaactc cgtgctgctg ttcctggcct tcgtggtgtt cctgctggtg accctggcta
      181 tcctgaccgc tctgagactg tgcgcttact gctgcaacat cgtgaacgtg tccctggtga
      241 agccctcttt ctacgtgtac agccgcgtga agaacctgaa cagctccagg gtgcctgacc
      301 tgctggtgta atacccaact ttcttgtaca aagttggcat tataagaaag cattgcttat
      361 caatttgttg caacgaacag gtcactatca gtcaaaataa aatcattatt tgccatccag
      421 ctgcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata
      481 tcatcatgaa caatanaact gtctgcttac ataaacagta atacaagggg tgttatgagc
      541 catattcaac gggaaacgtc gaggccgcga ttaaattcca acatggatgc tgatttatat
      601 gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaaatct atcgcttgta
      661 tgggaagccc gatgcgccag agttgtttct ganacntggc aaaggtagcg ttgccaatga
      721 tgttacagat gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat
      781 cnagcatttt atccgtactc ctgatga
//
