LOCUS       sequence-276451-         612 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector, with insert of the envelope (E) 
            gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 612)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554."
COMMENT     Sequence Label: pDONR207 SARS-CoV-2 E
FEATURES             Location/Qualifiers
     source          1..612
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    70..94
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(323..422)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      475..561
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 atgattttat tttgactgat agtgacctgt tcgttgcaac aaattgataa gcaatgcttt
       61 cttataatgc caactttgta caagaaagtt gggtattaca ccagcaggtc aggcaccctg
      121 gagctgttca ggttcttcac gcggctgtac acgtagaaag agggcttcac cagggacacg
      181 ttcacgatgt tgcagcagta agcgcacagt ctcagagcgg tcaggatagc cagggtcacc
      241 agcaggaaca ccacgaaggc caggaacagc agcacggagt tcacgatcag ggtgccggtt
      301 tcctcgctca cgaaagagta catgccaact tttttgtaca aagttggcat tataaaaaag
      361 cattgctcat caatttgttg caacgaacag gtcactatca gtcaaaataa aatcattatt
      421 tggggcccga gatccatgct agcgttaacg cgagagtagg gaactgccag gcatcaaata
      481 aaacgaaagg ctcagtcgga agactgggcc tttcgtttta tctgttgttt gtcggtgaac
      541 gctctcctga gtaggacaaa tccgccggga gcggatttga acgttgtgaa gcaacggccc
      601 ggagggtggc gg
//
