LOCUS       sequence-276452-        1008 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector, with insert of ORF3A gene's CDS 
            from SARS-CoV-2 isolate Wuhan-Hu-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554."
COMMENT     Sequence Label: pDONR207 SARS-CoV-2 ORF3A
FEATURES             Location/Qualifiers
     source          1..1008
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    70..94
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(923..947)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atgattttat tttgactgat agtgacctgt tcgttgcaac aaattgataa gcaatgcttt
       61 cttataatgc caactttgta caagaaagtt gggtattaca gtggcacgga ggtggtggtg
      121 gtaggttcgt cgtagatagg ctccatcacg gggttcacca cgccgctaga cacgtcgatg
      181 gtgtggatct gcacgtgttc ctcgggttcg tccacgatct tgttgtagat gaagaaggtc
      241 acgtgctcca caccggtgtc ggtagacagc tgggtggagt acagctggta gtagtcgctg
      301 gtgaagtaag agtgcagcac cacgcagtcc ttcactccag attcccactt ctcggtgtat
      361 ccgccgatct ggtagtcgtg ttcggagata gggctggtgg tgccgtcacc ggaggtgatc
      421 acgatggagc tggtcacgct gttgtagggg atgcagtagt cgtagcagtt ggtgtgccag
      481 cacaggaagt agttggcgtc gtacagcagg gggttcttgg atctgcactt ccagcacagc
      541 cacagcctca tgatgatgcg cacgaagttg atgctctgca ggaagtacac cagagcgtac
      601 aggtacagga atggagcctc cagaccagca gccaccagca gcaggtgtga gtacacggtc
      661 acgaacagca gcagcaggtt gcacacgaag tgcacaccct tggacagagc cagctgccac
      721 ctcttcttca gggtgatgat cttagaggcg gactggaaca cggccagcag ggccacaccc
      781 acgatcagcc atccgaaggg caggctagcc tggattggga tggtagcggt ggcacgcacg
      841 aagtcagaag gggtggcgtc cttgatctct ccctgcttca gggtcacggt gccgatggtg
      901 aagattctca tgaacaggtc catgccaact tttttgtaca aagttggcat tataaaaaag
      961 cattgctcat caatttgttg caacgaacag gtcactatca gtcaaaat
//
