LOCUS       sequence-276453-         980 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector, with insert of ORF7A gene's CDS 
            from SARS-CoV-2 isolate Wuhan-Hu-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554."
COMMENT     Sequence Label: pDONR207 SARS-CoV-2 ORF7A
FEATURES             Location/Qualifiers
     source          1..980
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    62..86
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(453..552)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(664..683)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(742..761)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 attttgactg atagtgacct gttcgttgca acaaattgat gagcaatgct tttttataat
       61 gccaactttg tacaaaaaag ttggcatgaa gatcatcctg ttcctggccc tgatcaccct
      121 ggctacctgc gagctgtacc actaccagga atgcgtgcgc ggtaccaccg tgctgctgaa
      181 ggagccttgc agctccggca cctacgaagg aaactccccc ttccaccctc tggccgacaa
      241 caagttcgct ctgacctgct tctctactca gttcgccttc gcttgccctg atggtgtgaa
      301 gcacgtgtac cagctgcgcg ccaggtctgt gagcccaaag ctgttcatca gacaggagga
      361 agtgcaggag ctgtacagcc ccatcttcct gatcgtggcc gctatcgtgt tcatcaccct
      421 gtgcttcacc ctgaagcgta agaccgaata atacccaact ttcttgtaca aagttggcat
      481 tataagaaag cattgcttat caatttgttg caacgaacag gtcactatca gtcaaaataa
      541 aatcattatt tgccatccag ctgcagctct ggcccgtgtc tcaaaatctc tgatgttaca
      601 ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac ataaacagta
      661 atacaagggg tgttatgagc catattcaac gggaaacgtc gaggccgcga ttaaattcca
      721 acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg
      781 cgacaatcta tcgcttgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca
      841 aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg ctgacggaat
      901 ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca
      961 ccactgcgat ccccggaaaa
//
