LOCUS       sequence-276456-         945 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector, with insert of ORF8 gene's CDS from
            SARS-CoV-2 isolate Wuhan-Hu-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554."
COMMENT     Sequence Label: pDONR223 SARS-CoV-2 ORF8
FEATURES             Location/Qualifiers
     source          1..945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    42..66
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(433..532)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(549..568)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(550..568)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(573..589)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(573..589)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag
       61 ttggcatgaa gttcctggtg ttcctgggta tcatcaccac cgtggccgct ttccaccagg
      121 agtgctctct gcagagctgc acccagcacc agccatacgt ggtggatgac ccatgcccta
      181 tccacttcta cagcaagtgg tacatcagag tgggagctag gaagtccgct cctctgatcg
      241 agctgtgcgt ggacgaagcc ggcagcaagt ccccaatcca gtacatcgac atcggaaact
      301 acaccgtgtc ttgcctgcca ttcaccatca actgccagga acccaagctg ggctccctgg
      361 tggtgagatg ctctttctac gaggacttcc tggaatacca cgacgtgcgt gtggtgctgg
      421 acttcatcta atacccaact ttcttgtaca aagttggcat tataagaaag cattgcttat
      481 caatttgttg caacgaacag gtcactatca gtcaaaataa aatcattatt tgccatccag
      541 ctgatatccc ctatagtgag tcgtattaca tggtcatagc tgtttcctgg cagctctggc
      601 ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca tcatgcctcc
      661 tctagaccag ccaggacaga aatgcctcga cttcgctgct acccaaggtt gccgggtgac
      721 gcacaccgtg gaaacggatg aaggcacgaa cccagtggac ataagcctgt tcggttcgta
      781 agctgtaatg caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca
      841 gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttggggtac
      901 agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgg
//
