LOCUS       sequence-276457-         951 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector, with insert of NSP1 gene's CDS from
            SARS-CoV-2 isolate Wuhan-Hu-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554."
COMMENT     Sequence Label: pDONR207 SARS-CoV-2 NSP1
FEATURES             Location/Qualifiers
     source          1..951
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    61..85
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(629..728)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(840..859)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(918..937)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 ttttgactga tagtgacctg ttcgttgcaa caaattgatg agcaatgctt ttttataatg
       61 ccaactttgt acaaaaaagt tggcatggag agcctggtgc ctggcttcaa cgaaaagacc
      121 cacgtgcagc tgtctctgcc agtgctgcag gtgcgcgacg tgctggtgag gggattcggt
      181 gactctgtgg aggaagtgct gtctgaagct agacagcacc tgaaggatgg tacctgcgga
      241 ctggtggagg tggaaaaggg cgtgctgccc cagctggagc agccttacgt gttcatcaag
      301 cgcagcgatg ctaggactgc tccccacgga cacgtgatgg tggaactggt ggccgagctg
      361 gaaggtatcc agtacggtcg ctccggagag accctgggtg tgctggtgcc acacgtgggt
      421 gaaatccccg tggcttacag aaaggtgctg ctgcgtaaga acggaaacaa gggtgccggc
      481 ggacactcct acggtgctga cctgaagtct ttcgacctgg gcgacgagct gggaactgac
      541 ccttacgagg acttccagga aaactggaac accaagcaca gctccggagt gaccagagag
      601 ctgatgcgtg aactgaacgg tggctaatac ccaactttct tgtacaaagt tggcattata
      661 agaaagcatt gcttatcaat ttgttgcaac gaacaggtca ctatcagtca aaataaaatc
      721 attatttgcc atccagctgc agctctggcc cgtgtctcaa aatctctgat gttacattgc
      781 acaagataaa aatatatcat catgaacaat aaaactgtct gcttacataa acagtaatac
      841 aaggggtgtt atgagccata ttcaacggga aacgtcgagg ccgcgattaa attccaacat
      901 ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat c
//
