LOCUS       sequence-276461-         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector, with insert of NSP7 gene's CDS from
            SARS-CoV-2 isolate Wuhan-Hu-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554."
COMMENT     Sequence Label: pDONR223 SARS-CoV-2 NSP7
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    68..92
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(348..447)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(464..483)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(465..483)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(488..504)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(488..504)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gattttattt tgactgatag tgacctgttc gttgcaacaa attgatgagc aatgcttttt
       61 tataatgcca actttgtaca aaaaagttgg catgagcaag atgtccgacg tgaagtgcac
      121 ctccgtggtg ctgctgtctg tgctgcagca gctgcgcgtg gagagctcct ctaagctgtg
      181 ggcccagtgc gtgcagctgc acaacgacat cctgctggcc aaggacacca ccgaggcttt
      241 cgaaaagatg gtgtctctgc tgagcgtgct gctgtccatg cagggagctg tggacatcaa
      301 caagctgtgc gaggaaatgc tggacaacag ggctaccctg cagtaatacc caactttctt
      361 gtacaaagtt ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac
      421 tatcagtcaa aataaaatca ttatttgcca tccagctgat atcccctata gtgagtcgta
      481 ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta
      541 cattgcacaa gataaaaata tatcatcatg cctcctctag accagccagg acagaaatgc
      601 ctcgacttcg ctgctaccca aggttgccgg gtgacgcaca ccgtggaaac ggatgaaggc
      661 acgaacccag tggacataag cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg
      721 ctcacgcaac tggtccagaa ccttgacnga acgcagcggt ggtaacggcg cantggcggt
      781 tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg catccaagca
      841 gcaagcgcgt tacgccgtgg gtcgatgttt gatgt
//
