LOCUS       sequence-276462-         659 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector, with insert of NSP7 gene's CDS from
            SARS-CoV-2 isolate Wuhan-Hu-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 659)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554."
COMMENT     Sequence Label: pDONR223 SARS-CoV-2 NSP7
FEATURES             Location/Qualifiers
     source          1..659
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    12..111
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(367..466)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(482..499)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(482..498)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(491..513)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      562..648
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 cagctggatg gcaaataatg attttatttt gactgatagt gacctgttcg ttgcaacaaa
       61 ttgataagca atgctttctt ataatgccaa ctttgtacaa gaaagttggg tattactgca
      121 gggtagccct gttgtccagc atttcctcgc acagcttgtt gatgtccaca gctccctgca
      181 tggacagcag cacgctcagc agagacacca tcttttcgaa agcctcggtg gtgtccttgg
      241 ccagcaggat gtcgttgtgc agctgcacgc actgggccca cagcttagag gagctctcca
      301 cgcgcagctg ctgcagcaca gacagcagca ccacggaggt gcacttcacg tcggacatct
      361 tgctcatgcc aacttttttg tacaaagttg gcattataaa aaagcattgc tcatcaattt
      421 gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttggggc ccgagcttaa
      481 gactggccgt cgttttacaa cgtcgtgact gggaaancat ccatgctagc gttaacgcga
      541 gagtanggaa ctgccaggca tcaaataaaa cgaaaggctc actcggaaga ctgggccttt
      601 cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgggagc
//
