LOCUS       sequence-276465-        1020 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector, with insert of NSP13 gene's CDS 
            from SARS-CoV-2 isolate Wuhan-Hu-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554."
COMMENT     Sequence Label: pDONR223 SARS-CoV-2 NSP13
FEATURES             Location/Qualifiers
     source          1..1020
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    68..92
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gattttattt tgactgatag tgacctgttc gttgcaacaa attgatgagc aatgcttttt
       61 tataatgcca actttgtaca aaaaagttgg catggccgtg ggcgcttgcg tgctgtgcaa
      121 ctctcagacc agcctgagat gtggagcttg catccgcagg cctttcctgt gctgcaagtg
      181 ctgctacgac cacgtgatca gcacctccca caagctggtg ctgtccgtga acccatacgt
      241 gtgcaacgct cctggttgtg acgtgactga cgtgacccag ctgtacctgg gcggaatgag
      301 ctactactgc aagtcccaca agccccctat cagcttccca ctgtgcgcta acggccaggt
      361 gttcggactg tacaagaaca cctgcgtggg ctccgacaac gtgaccgact tcaacgccat
      421 cgctacctgc gactggacca acgccggaga ctacatcctg gctaacacct gcaccgaaag
      481 gctgaagctg ttcgccgctg agaccctgaa ggccaccgag gaaaccttca agctgtctta
      541 cggtatcgct accgtgcgtg aggtgctgtc tgaccgcgaa ctgcacctgt cttgggaagt
      601 gggcaagcca agaccacccc tgaaccgtaa ctacgtgttc accggataca gggtgaccaa
      661 gaacagcaag gtgcagatcg gagagtacac cttcgaaaag ggagactacg gtgacgccgt
      721 ggtgtacaga ggaaccacca cctacaagct gaacgtgggt gactacttcg tgctgacctc
      781 ccacaccgtg atgccactgt ctgctcccac cctggtgcct caggaacact acgtgcgcat
      841 caccggactg tacccaaccc tgaacatctc cgacgagttc agctccaacg tggccaacta
      901 ccagaaagtg ggcatgcaga agtactccac cctgcaggga cctccaggta ccggcaagtc
      961 tcacttcgcc atcggtctgg ctctgtacta cccctctgcc cgtatcgtgt acactgcttg
//
