LOCUS       sequence-276467-         903 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector, with insert of NSP8 gene's CDS from
            SARS-CoV-2 isolate Wuhan-Hu-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554."
COMMENT     Sequence Label: pDONR223 SARS-CoV-2 NSP8
FEATURES             Location/Qualifiers
     source          1..903
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    73..97
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(698..797)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(813..830)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(813..829)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(822..844)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc
       61 tttcttataa tgccaacttt gtacaagaaa gttgggtatt actgcagctt cacggcagag
      121 ttagcgcgca gggcggtcac gatcagtggc caagccaggt ttgggctgtt gtccatgctg
      181 atttcagaca gctgcacgat cttagagtca gcgtccacca cctgctggat ctcccacaga
      241 gcggaggcgt aggtgaaggt ggttccgtcg caggtgttct tgtaggtgtt gtagtcgggg
      301 atgaccacca tcagcttagc ggcggtggtc agagggatga tgttcagggg cacgcagccg
      361 tctctggcgt tgttgatgat gttgttcaga gcgtcgttgt ccagcttgcg cagcatggtg
      421 aacagcatgg tctgcatggc gctggtcacc ttagccctct tgtcctcaga gcgagcctgc
      481 ttgtacatct gggtcatagc ctggtcggcc atcttttcca gcttacgctg catagcggcg
      541 tctctgtcga actcagactt ggccacgttc agggacttct tcagcttctt cagcaccact
      601 tcggagtcgc cgttagccac ggcctgttcg taagcctcct gagcggtggc gaaagcggcg
      661 taggaaggca gggagctgaa ctcgctagcg atggccatgc caactttttt gtacaaagtt
      721 ggcattataa aaaagcattg ctcatcaatt tgttgcaacg aacaggtcac tatcagtcaa
      781 aataaaatca ttatttgggg cccgagctta agactggccg tcgttttaca acgtcgtgac
      841 tgggaaaaca tccatgctag cgttaacgcg agagtaggga actgccaggc atcaaataaa
      901 acg
//
