LOCUS       sequence-276468-         979 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector, with insert of NSP8 gene's CDS from
            SARS-CoV-2 isolate Wuhan-Hu-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554."
COMMENT     Sequence Label: pDONR223 SARS-CoV-2 NSP8
FEATURES             Location/Qualifiers
     source          1..979
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    68..92
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(693..792)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(809..828)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(810..828)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(833..849)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(833..849)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gattttattt tgactgatag tgacctgttc gttgcaacaa attgatgagc aatgcttttt
       61 tataatgcca actttgtaca aaaaagttgg catggccatc gctagcgagt tcagctccct
      121 gccttcctac gccgctttcg ccaccgctca ggaggcttac gaacaggccg tggctaacgg
      181 cgactccgaa gtggtgctga agaagctgaa gaagtccctg aacgtggcca agtctgagtt
      241 cgacagagac gccgctatgc agcgtaagct ggaaaagatg gccgaccagg ctatgaccca
      301 gatgtacaag caggctcgct ctgaggacaa gagggctaag gtgaccagcg ccatgcagac
      361 catgctgttc accatgctgc gcaagctgga caacgacgct ctgaacaaca tcatcaacaa
      421 cgccagagac ggctgcgtgc ccctgaacat catccctctg accaccgccg ctaagctgat
      481 ggtggtcatc cccgactaca acacctacaa gaacacctgc gacggaacca ccttcaccta
      541 cgcctccgct ctgtgggaga tccagcaggt ggtggacgct gactctaaga tcgtgcagct
      601 gtctgaaatc agcatggaca acagcccaaa cctggcttgg ccactgatcg tgaccgccct
      661 gcgcgctaac tctgccgtga agctgcagta atacccaact ttcttgtaca aagttggcat
      721 tataagaaag cattgcttat caatttgttg caacgaacag gtcactatca gtcaaaataa
      781 aatcattatt tgccatccag ctgatatccc ctatagtgag tcgtattaca tggtcatagc
      841 tgtttcctgg cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa
      901 aaatatatca tcatgcctcc tctagaccag ccaggacaga aatgcctcga cttcgctgct
      961 acccaaggtt gccgggtga
//
