LOCUS       sequence-276475-         788 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector, with insert of NSP9 gene's CDS from
            SARS-CoV-2 isolate Wuhan-Hu-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554."
COMMENT     Sequence Label: pDONR223 SARS-CoV-2 NSP9
FEATURES             Location/Qualifiers
     source          1..788
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    12..111
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(457..556)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(572..589)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(572..588)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(581..603)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      652..738
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 cagctggatg gcaaataatg attttatttt gactgatagt gacctgttcg ttgcaacaaa
       61 ttgataagca atgctttctt ataatgccaa ctttgtacaa gaaagttggg tattactgca
      121 gcctcacggt agcagccagg ctacccagca ccattcctct gttcaggttg ttcagtccct
      181 tgatgaagta caggtacttc accttggggc cctttggggt gtcggtcacg aatctgcaag
      241 ggggttccag ctcggtgtag atggtgccgg taccgtcgct cttggggaaa cgagcccact
      301 tcaggtcctg caggtcagac agcagggcca gcacgaatct tccgcccttg gtggtgttgt
      361 agtaagccag ggcgttgtcg tcggtgcaag cggtctgggt ggtaccagca gcacaagaca
      421 tctgtctcag ggccacaggg ctcagctcgt tgttcatgcc aacttttttg tacaaagttg
      481 gcattnnnaa aaagcattgc tcatcaattt gttgcaacga acaggtcact atcagtcaaa
      541 ataaaatcat tatttggggc ccgagcttaa gactggccgt cgttttacaa cgtcgtgact
      601 gggaaaacat ccatgctagc gttaacgcga gagtagggaa ctgccaggca tcaaataaaa
      661 cgaaaggctc agtcggaaga ctgggccttt cgttttatct gttgtttgtc ngtgaacgct
      721 ctcctgagta ggacaaatcc gccgggagcg gatttgaacg ttgtgaagca acggcccgga
      781 gggtggcg
//
