LOCUS       sequence-27649-e         710 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 710)
  TITLE     CHOP plasmids
REFERENCE   2  (bases 1 to 710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 9E10.mCHOP(S78&81A).pCDNA1/Amp
FEATURES             Location/Qualifiers
     source          1..710
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             103..132
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     promoter        complement(691..709)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(692..709)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagcttgg taccgagctc ggatccgcca tggagcaaaa gctgatttct
      121 gaggaggatc tgggaggggc agctgagtcc ctgcctttca ccttggagac ggtgtccagc
      181 tgggagctgg aagcctggta tgaggatctg caggaggtcc tgtcctcaga tgaaattggg
      241 ggcacctata tctcatcccc aggaaacgaa gaggaagaat caaaaacctt cactactctt
      301 gaccctgcgt ccctagcttg gctgacagag gagccagggc caacagaggt cacacgcaca
      361 tcccaagccc ctcgcgctcc agattccagt cagagttcta tggcccagga ggaagaggag
      421 gaagagcaag gaagaactag gaaacggaaa cagagtggtc agtgcccagc ccggcctggg
      481 aagcaacgca tgaaggagaa ggagcaggag aacgagcgga aagtggcaca gctagctgaa
      541 gagaacgagc ggctcaagca ggaaatcgag cgcctgacca gggaggtgga gaccacacgg
      601 cgggctctga tcgaccgcat ggtcagcctg caccaagcat gaacagtggg catcacctcg
      661 acctgcaggc atgcatctag agggccctat tctatagtgt cacctaaatg
//
