LOCUS       sequence-276512-        6878 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Prey vector ERL1 C11_pECIA14 should be used with bait vector ERL1 
            C11_pECIA2..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6878)
  AUTHORS   Smakowska-Luzan E, Mott GA, Parys K, Stegmann M, Howton TC, 
            Layeghifard M, Neuhold J, Lehner A, Kong J, Grunwald K, Weinberger 
            N, Satbhai SB, Mayer D, Busch W, Madalinski M, Stolt-Bergner P, 
            Provart NJ, Mukhtar MS, Zipfel C, Desveaux D, Guttman DS, Belkhadir 
            Y
  TITLE     An extracellular network of Arabidopsis leucine-rich repeat receptor
            kinases.
  JOURNAL   Nature. 2018 Jan 18;553(7688):342-346. doi: 10.1038/nature25184. 
            Epub 2018 Jan 10.
  PUBMED    29320478
REFERENCE   2  (bases 1 to 6878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature25184"; journalName: "Nature"; date: "2018-01-18- 
            18"; volume: "553"; issue: "7688"; pages: "342-346"
COMMENT     Sequence Label: ERL1 C11_pECIA14
FEATURES             Location/Qualifiers
     source          1..6878
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        23..449
                     /label=MT promoter
                     /note="Drosophila metallothionein promoter"
     sig_peptide     467..520
                     /codon_start=1
                     /product="Drosophila BiP signal sequence"
                     /label=BiP signal sequence
                     /translation="MKLCILLAVVAFVGLSLG"
     CDS             2174..2197
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             3872..3895
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3911..3928
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3951..3968)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4001..4135
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4025..4044)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4079..4098
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(4279..4295)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4279..4295)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4292..4314)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4303..4319
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4327..4333,4334..4351,4352..4357))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4372..4393
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4510..4527)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4681..5269)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4761..4780)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5440..6231,6232..6300))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6063..6082
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6301..6405)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6473..6491
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6529..6551)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6651..6670
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     join(6864..6878,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gtaaaacgac ggccagtgaa ttaattcgtt gcaggacagg atgtggtgcc cgatgtgact
       61 agctctttgc tgcaggccgt cctatcctct ggttccgata agagacccag aactccggcc
      121 ccccaccgcc caccgccacc cccatacata tgtggtacgc aagtaagagt gcctgcgcat
      181 gccccatgtg ccccaccaag agctttgcat cccatacaag tccccaaagt ggagaaccga
      241 accaattctt cgcgggcaga acaaaagctt ctgcacacgt ctccactcga atttggagcc
      301 ggccggcgtg tgcaaaagag gtgaatcgaa cgaaagaccc gtgtgtaaag ccgcgtttcc
      361 aaaatgtata aaaccgagag catctggcca atgtgcatca gttgtggtca gcagcaaaat
      421 caagtgaatc atctcagtgc aactaaaggg gggatccgat ctcaatatga agttatgcat
      481 attactggcc gtcgtggcct ttgttggcct ctcgctcggg atgaacaacg aagggaaagc
      541 tctgatggcg ataaaaggct ctttcagcaa cttagtgaat atgcttttgg attgggacga
      601 tgttcacaac agtgacttgt gttcttggcg aggtgttttc tgcgacaacg ttagctactc
      661 cgttgtctct ctgaatttgt ccagtctgaa tcttggaggg gagatatctc cagctattgg
      721 agacctacgg aatttgcaat caatagactt gcaaggtaat aaactagcag gtcaaattcc
      781 agatgagatt ggaaactgtg cttctcttgt ttatctggat ttgtccgaga atctgttata
      841 tggagacata cctttctcaa tctctaaact caagcagctt gaaactctga atctgaagaa
      901 caatcagctc acaggtcctg taccagcaac cttaacccag attccaaacc ttaagagact
      961 tgatcttgct ggcaatcatc taacgggtga gatatcgaga ttgctttact ggaatgaagt
     1021 tttgcagtat cttggattac gagggaatat gttgactgga acgttatctt ctgatatgtg
     1081 tcagctaacc ggtttgtggt actttgatgt gagaggaaat aatctaactg gaaccatccc
     1141 ggagagcatc ggaaattgca caagctttca aatcctggac atatcttata atcagataac
     1201 aggagagatt ccttacaata tcggcttcct ccaagttgct actctgtcac ttcaaggaaa
     1261 cagattgacg ggtagaattc cagaagttat tggtctaatg caggctcttg ctgttttgga
     1321 tttgagtgac aatgagcttg ttggtcctat cccaccgata cttggcaatc tctcatttac
     1381 cggaaagttg tatctccatg gcaatatgct cactggtcca atcccctctg agcttgggaa
     1441 tatgtcacgt ctcagctatt tgcagctaaa cgacaataaa ctagtgggaa ctattccacc
     1501 tgagcttgga aagctggagc aattgtttga actgaatctt gccaacaacc gtttagtagg
     1561 gcccatacca tccaacatta gttcatgtgc agccttgaat caattcaatg ttcatgggaa
     1621 cctcttgagt ggatctattc cactggcgtt tcgcaatctc gggagcttga cttatctgaa
     1681 tctttcgtcg aacaatttca agggaaaaat accagttgag cttggacata taatcaatct
     1741 tgacaaacta gatctgtctg gcaataactt ctcagggtct ataccattaa cgcttggcga
     1801 tcttgaacac cttctcatat taaatcttag cagaaaccat cttagtggac aattacctgc
     1861 agagtttggg aaccttcgaa gcattcagat gattgatgta tcattcaatc tgctctccgg
     1921 agttattcca actgaacttg gccaattgca gaatttaaac tctttaatat tgaacaacaa
     1981 caagcttcat gggaaaattc cagatcagct tacgaactgc ttcactcttg tcaatctgaa
     2041 tgtctccttc aacaatctct ccgggatagt cccaccaatg aaaaacttct cacgttttgc
     2101 tccagccagc tttgttggaa atccatatct ttgtggaaac tgggttggat ctatttgtgg
     2161 tcctttaccg aaactggagg tgctgttcca gggaccaagc ggcaatcccg gcggcgatct
     2221 ggccccccag atgctgcgcg agctgcagga gaccaatgcc gccctgcagg acgtgcgcga
     2281 gctgctgcgc caccgcgtga aggagatcac cttcctgaag aacaccgtga tggagtgcga
     2341 cgcctgcggc atgcagccct ctagaggctc cggctccatc atcccagttg aggaggagaa
     2401 cccggacttc tggaaccgcg aggcagccga ggccctgggt gccgccaaga agctgcagcc
     2461 tgcacagaca gccgccaaga acctcatcat cttcctgggc gatgggatgg gggtgtctac
     2521 ggtgacagct gccaggatcc taaaagggca gaagaaggac aaactggggc ctgagatacc
     2581 cctggccatg gaccgcttcc catatgtggc tctgtccaag acatacaatg tagacaaaca
     2641 tgtgccagac agtggagcca cagccacggc ctacctgtgc ggggtcaagg gcaacttcca
     2701 gaccattggc ttgagtgcag ccgcccgctt taaccagtgc aacacgacac gcggcaacga
     2761 ggtcatctcc gtgatgaatc gggccaagaa agcagggaag tcagtgggag tggtaaccac
     2821 cacacgagtg cagcacgcct cgccagccgg cacctacgcc cacacggtga accgcaactg
     2881 gtactcggac gccgacgtgc ctgcctcggc ccgccaggag gggtgccagg acatcgctac
     2941 gcagctcatc tccaacatgg acattgacgt gatcctaggt ggaggccgaa agtacatgtt
     3001 tcgcatggga accccagacc ctgagtaccc agatgactac agccaaggtg ggaccaggct
     3061 ggacgggaag aatctggtgc aggaatggct ggcgaagcgc cagggtgccc ggtatgtgtg
     3121 gaaccgcact gagctcatgc aggcttccct ggacccgtct gtgacccatc tcatgggtct
     3181 ctttgagcct ggagacatga aatacgagat ccaccgagac tccacactgg acccctccct
     3241 gatggagatg acagaggctg ccctgcgcct gctgagcagg aacccccgcg gcttcttcct
     3301 cttcgtggag ggtggtcgca tcgaccatgg tcatcatgaa agcagggctt accgggcact
     3361 gactgagacg atcatgttcg acgacgccat tgagagggcg ggccagctca ccagcgagga
     3421 ggacacgctg agcctcgtca ctgccgacca ctcccacgtc ttctccttcg gaggctaccc
     3481 cctgcgaggg agctccatct tcgggctggc ccctggcaag gcccgggaca ggaaggccta
     3541 cacggtcctc ctatacggaa acggtccagg ctatgtgctc aaggacggcg cccggccgga
     3601 tgttaccgag agcgagagcg ggagccccga gtatcggcag cagtcagcag tgcccctgga
     3661 cgaagagacc cacgcaggcg aggacgtggc ggtgttcgcg cgcggcccgc aggcgcacct
     3721 ggttcacggc gtgcaggagc agaccttcat agcgcacgtc atggccttcg ccgcctgcct
     3781 ggagccctac accgcctgcg acctggcgcc ccccgccggc accaccgacg ccgcgcaccc
     3841 gggttctaga ggaagcacct ccggcgataa cgattacaag gatgatgatg ataaggccag
     3901 cggcaccggt catcatcacc atcaccattg agtttaaacc cgctgatcag cctcgactgt
     3961 gccttctaag gcctgagctc gctgatcagc ctcgatcgag gatccagaca tgataagata
     4021 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
     4081 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
     4141 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag
     4201 caagtaaaac ctctacaaat gtggtatggc tgattatgat cagtcgacct gcaggcatgc
     4261 aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     4321 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     4381 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     4441 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     4501 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     4561 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     4621 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     4681 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     4741 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     4801 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     4861 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     4921 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     4981 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     5041 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     5101 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     5161 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     5221 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     5281 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     5341 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     5401 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     5461 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     5521 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     5581 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     5641 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     5701 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     5761 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     5821 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     5881 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     5941 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     6001 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     6061 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     6121 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     6181 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     6241 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     6301 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     6361 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     6421 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     6481 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
     6541 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
     6601 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga
     6661 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag
     6721 aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc
     6781 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt
     6841 aagttgggta acgccagggt tttcccagtc acgacgtt
//
