LOCUS       sequence-27654-e         769 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 769)
  TITLE     CHOP plasmids
REFERENCE   2  (bases 1 to 769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CHOP 6: mCHOP-WT-9E10-pcDNA1
FEATURES             Location/Qualifiers
     source          1..769
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             106..135
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     promoter        complement(687..705)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(688..705)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct tggtaccgag ctcggatccg ccatggagca aaagctgatt
      121 tctgaggagg atctgggagg ggcagctgag tccctgcctt tcaccttgga gacggtgtcc
      181 agctgggagc tggaagcctg gtatgaggat ctgcaggagg tcctgtcctc agatgaaatt
      241 gggggcacct atatctcatc cccaggaaac gaagaggaag aatcaaaaac cttcactact
      301 cttgaccctg cgtccctagc ttggctgaca gaggagccag ggccaacaga ggtcacacgc
      361 acatcccaaa gccctcgctc tccagattcc agtcagagtt ctatggccca ggaggaagag
      421 gaggaagagc aaggaagaac taggaaacgg aaacagagtg gtcagtgccc agcccggcct
      481 gggaagcaac gcatgaagga gaaggagcag gagaacgagc ggaaagtggc acagctagct
      541 gaagagaacg agcggctcaa gcaggaaatc gagcgcctga ccagggaggt ggagaccaca
      601 cggcgggctc tgatcgaccg catggtcagc ctgcaccaag catgaacagt gggcatcacc
      661 tcgagcatgc atctagaggg ccctattcta tagtgtcacc taaatgctag aggatctttg
      721 tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctac
//
