LOCUS       sequence-27656-e         755 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 755)
  TITLE     ATF4 plasmids
REFERENCE   2  (bases 1 to 755)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ATF4 2: Mouse ATF4 (CHOP11/cATF)-bZIP
FEATURES             Location/Qualifiers
     source          1..755
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             108..137
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(559..575)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(560..576)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(590..608)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(591..608)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(628..645)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    634..744
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 cnganctctc tggctaacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac
       61 tcactatagg gagacccaag cttggtaccg agctcggatc cgccatggag caaaagctga
      121 tttctgagga ggatctgggg tctcctcggc ccaaacctta tgacccacct ggagttagtt
      181 tgacagctaa agtgaagact gagaaattgg ataagaagct gaaaaagatg gagcaaaaca
      241 agacagcagc cactaggtac cgccagaaga agcgggctga gcaggaggcc ctcactggcg
      301 agtgtaagga gctagaaaaa aagaatgagg ctctgaaaga gaaggcagat tctctggcca
      361 aggagatcca gtatctgaaa gacctgatag aagaggtccg taaggcaagg gggaagaaga
      421 gagttccgta atagggtagt caggtgcttt gtgcttgtac atagtcttgt gttgctgtgt
      481 ttgctgtaat aaattatttt gtagtgaaag taaaaaaaaa aaaaaaaaaa ctcgtgccga
      541 attcgatatc aagcttatcg ataccgtcga cctcgagggg gggccctatt ctatagtgtc
      601 acctaaatgc tagagctcgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
      661 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
      721 cctaataaan gaggaaattg catcgcattg tctga
//
