LOCUS       sequence-276592-        2438 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing the SP1 transcription factor 
            ORF with a unique 24-bp barcode. Barcodes facilitate identification 
            of transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0033
FEATURES             Location/Qualifiers
     source          1..2438
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggatgaaat gacagctgtg gtgaaaattg aaaaaggagt tggtggcaat
       61 aatgggggca atggtaatgg tggtggtgcc ttttcacagg ctcgaagtag cagcacaggc
      121 agtagcagca gcactggagg aggagggcag gagtcccagc catccccttt ggctctgctg
      181 gcagcaactt gcagcagaat tgagtcaccc aatgagaaca gcaacaactc ccagggcccg
      241 agtcagtcag ggggaacagg tgagcttgac ctcacagcca cacaactttc acagggtgcc
      301 aatggctggc agatcatctc ttcctcctct ggggctaccc ctacctcaaa ggaacagagt
      361 ggcagcagta ccaatggcag caatggcagt gagtcttcca agaatcgcac agtctctggt
      421 gggcagtatg ttgtggctgc cgctcccaac ttacagaacc agcaagttct gacaggacta
      481 cctggagtga tgcctaatat tcagtatcaa gtaatcccac agttccagac cgttgatggg
      541 caacagctgc agtttgctgc cactggggcc caagtgcagc aggatggttc tggtcaaata
      601 cagatcatac caggtgcaaa ccaacagatt atcacaaatc gaggaagtgg aggcaacatc
      661 attgctgcta tgccaaacct actccagcag gctgtccccc tccaaggcct ggctaataat
      721 gtactctcag gacagactca gtatgtgacc aatgtaccag tggccctgaa tgggaacatc
      781 accttgctac ctgtcaacag cgtttctgca gctaccttga ctcccagctc tcaggcagtc
      841 acgatcagca gctctgggtc ccaggagagt ggctcacagc ctgtcacctc agggactacc
      901 atcagttctg ccagcttggt atcatcacaa gccagttcca gctccttttt caccaatgcc
      961 aatagctact caactactac taccaccagc aacatgggaa ttatgaactt tactaccagt
     1021 ggatcatcag ggaccaactc tcaaggccag acaccccaga gggtcagtgg gctacagggg
     1081 tctgatgctc tgaacatcca gcaaaaccag acatctggag gctcattgca agcaggccag
     1141 caaaaagaag gagagcaaaa ccagcagaca cagcagcaac aaattcttat ccagcctcag
     1201 ctagttcaag ggggacaggc cctccaggcc ctccaagcag caccattgtc agggcagacc
     1261 tttacaactc aagccatctc ccaggaaacc ctccagaacc tccagcttca ggctgttcca
     1321 aactctggtc ccatcatcat ccggacacca acagtggggc ccaatggaca ggtcagttgg
     1381 cagactctac agctgcagaa cctccaagtt cagaacccac aagcccaaac aatcacctta
     1441 gccccaatgc agggtgtttc cttggggcag accagcagca gcaacaccac tctcacaccc
     1501 attgcctcag ctgcttccat tcctgctggc acagtcactg tgaatgctgc tcaactctcc
     1561 tccatgccag gcctccagac cattaacctc agtgcattgg gtacttcagg aatccaggtg
     1621 cacccaattc aaggcctgcc gttggctata gcaaatgccc caggtgatca tggagctcag
     1681 cttggtctcc atggggctgg tggtgatgga atacatgatg acacagcagg tggagaggaa
     1741 ggagaaaaca gcccagatgc ccaaccccaa gccggtcgga ggacccggcg ggaagcatgc
     1801 acctgcccct actgtaaaga cagtgaagga aggggctcgg gggatcctgg caaaaagaaa
     1861 cagcatattt gccacatcca aggctgtggg aaagtgtatg gcaagacctc tcacctgcgg
     1921 gcacacttgc gctggcatac aggcgagagg ccatttatgt gtacctggtc atactgtggg
     1981 aaacgcttca cacgttcgga tgagctacag aggcacaaac gtacacacac aggtgagaag
     2041 aaatttgcct gccctgagtg tcctaagcgc ttcatgagga gtgaccacct gtcaaaacat
     2101 atcaagaccc accagaataa gaagggaggc ccaggtgtag ctctgagtgt gggcactttg
     2161 cccctggaca gtggggcagg ttcagaaggc agtggcactg ccactccttc agcccttatt
     2221 accaccaata tggtagccat ggaggccatc tgtccagagg gcattgcccg tcttgccaac
     2281 agtggcatca acgtcatgca ggtggcagat ctgcagtcca ttaatatcag tggcaatggc
     2341 ttctagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
     2401 tgtggaaagg acgactttat atttcggcgg ccgagcag
//
