LOCUS       sequence-27661-e         727 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Lu PD, Harding HP, Ron D
  TITLE     Translation reinitiation at alternative open reading frames 
            regulates gene expression in an integrated stress response.
  JOURNAL   J Cell Biol. 2004 Oct 11. 167(1):27-33.
  PUBMED    15479734
REFERENCE   2  (bases 1 to 727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2004 Oct 11. 167(1):27-33."
COMMENT     Sequence Label: ATF4 8: (SL-)5'ATF4.GFP
FEATURES             Location/Qualifiers
     source          1..727
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      185..194
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      522..531
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(573..594)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagctt ggtaccgggc cccccctcga gctcaagctt ttctgcttgc
      121 tgtctgccgg tttgagttgt gcgctcgggt gtccctttcc tcttcccctc ccgcagggct
      181 tgcggccacc atggcgtatt agaggcagca gtgcctgcgg cagcgttggc ctttgcagcg
      241 gcggcagcag caccaggctc tgcagcggca acccccaccg gcctaagcca tggcgctctt
      301 cacgaaatcc agcagcagtg ttgctgtaac ggacaaagat accttcgagt taagcacatt
      361 cctggaatcc agcaaagccc cacaacatga ccgagatgag cttcctgaac agcgaagtgt
      421 tggcggggga cttgatgtcc cccttcgacc agtcgggttt gggggctgaa gaaagcctag
      481 gtctcttaga tgactatctg gaggtggggg atccaccggt cgccaccatg gtgagcaagg
      541 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
      601 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
      661 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
      721 tgaccta
//
