LOCUS       sequence-27665-e         766 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 766)
  AUTHORS   Lu PD, Harding HP, Ron D
  TITLE     Translation reinitiation at alternative open reading frames 
            regulates gene expression in an integrated stress response.
  JOURNAL   J Cell Biol. 2004 Oct 11. 167(1):27-33.
  PUBMED    15479734
REFERENCE   2  (bases 1 to 766)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2004 Oct 11. 167(1):27-33."
COMMENT     Sequence Label: ATF4 11: 5'ATF4.uORF2.GFP
FEATURES             Location/Qualifiers
     source          1..766
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      138..147
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    425..477
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      489..498
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(540..561)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 agctggttta gtgaaccgtc agatccgcta gcgctaccgg actcagatct cgagctcaag
       61 cttttctgct tgctgtctgc cggtttgagt tgtgcgctcg ggtgtccctt tcctcttccc
      121 ctcccgcagg gcttgcggcc accatggcgt attagaggca gcagtgcctg cggcagcgtt
      181 ggcctttgca gcggcggcag cagcaccagg ctctgcagcg gcaaccccca ccggcctaag
      241 ccatggcgct cttcacgaaa tccagcagca gtgttgctgt aacggacaaa gataccttcg
      301 agttaagcac attcctggaa tccagcaaag ccccacaaca tgaccgagat gagcttcctg
      361 aacagcgaag tgttggcggg ggacttgatg tcccccttcg accagtcggg tttgggggct
      421 ggatctcgag ctcaagcttc gaattctgca gtcgacggta ccgcgggccc gggatccacc
      481 ggccggtcgc caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
      541 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
      601 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
      661 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc
      721 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccga
//
