LOCUS       sequence-276655-        1670 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing the ZNF454 transcription factor
            ORF with a unique 24-bp barcode. Barcodes facilitate identification 
            of transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0073
FEATURES             Location/Qualifiers
     source          1..1670
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggctgtcag ccacctgcca accatggtcc aggaatcggt gaccttcaag
       61 gatgtggcta tactgttcac ccaggaagag tgggggcagc tgagccccgc ccagagggcc
      121 ctgtacaggg acgtgatgct ggagaactac agcaacctgg tctcactggg actcttagga
      181 cccaaaccag atacgttttc ccagctagaa aaaagggaag tgtggatgcc agaggacacc
      241 cctggaggct tctgtcttga ctggatgact atgcctgcca gtaagaaatc tactgtcaag
      301 gcagagattc ctgaagaaga attggatcaa tggacaataa aggaaagatt cagtagcagt
      361 agtcactgga agtgtgctag cctgctggag tggcaatgtg gaggccagga gatcagtttg
      421 cagcgagtgg tactcactca ccccaacacc ccatcacagg aatgtgatga atccgggagc
      481 actatgagct catctcttca cagtgatcaa agtcagggat ttcaacctag caaaaatgcc
      541 tttgagtgta gtgagtgtgg aaaagtcttc tctaagagtt caactcttaa taaacatcag
      601 aaaattcata atgaaaaaaa tgcaaatcag aaaattcata ttaaggagaa aagatatgaa
      661 tgtagagaat gtgggaaagc ctttcaccag agtacgcacc ttatccatca ccaaagaatt
      721 cacactggcg agaaacccta tgaatgtaag gaatgtggca aggccttctc agtgagctcc
      781 tcacttacgt accatcagaa aattcatact ggagagaagc cttttgaatg caacttatgt
      841 ggaaaagctt ttatccgaaa tatacacctt gcccatcatc atagaataca tactggagag
      901 aaacctttta aatgtaacat ttgtgaaaaa gcctttgtgt gcagggcaca ccttaccaaa
      961 caccagaata tccacagtgg agagaaaccc tataaatgca atgaatgtgg aaaagccttt
     1021 aatcagagta caagtttcct tcagcatcag agaattcaca ctggagagaa accctttgaa
     1081 tgtaatgaat gtgggaaggc cttcagggtg aactcttccc ttactgaaca tcagagaatt
     1141 catactggag agaaacctta taaatgtaat gaatgtggga aagctttcag ggataattca
     1201 tcctttgcac gacatcggaa aattcacact ggagagaaac cttacagatg tggcttgtgt
     1261 gagaaagcct ttcgggacca atcagcacta gcccaacatc agagaattca tactggggaa
     1321 aaaccttata catgtaacat atgtgaaaaa gccttcagtg accattcagc ccttacccaa
     1381 cataagagaa ttcatactag ggaaaaacct tacaaatgta aaatctgtga gaaagccttt
     1441 atccgaagca ctcacctgac tcaacatcag aggattcaca caggagagaa accctataaa
     1501 tgtaataaat gtgggaaagc ttttaaccag actgcaaacc tcattcagca tcagagacat
     1561 catattggag agaagtagta atgaactagt ccgtaacttg aaagtatttc gatttcttgg
     1621 ctttatatat cttgtggaaa ggacgatttg acccaggacg gctatatcca
//
