LOCUS       sequence-276670-         731 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing the LIN28A transcription factor
            ORF with a unique 24-bp barcode. Barcodes facilitate identification 
            of transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0087
FEATURES             Location/Qualifiers
     source          1..731
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggctccgt gtccaaccag cagtttgcag gtggctgcgc caaggcggca
       61 gaagaggcgc ccgaggaggc gccggaggac gcggcccggg cggcggacga gcctcagctg
      121 ctgcacggtg cgggcatctg taagtggttc aacgtgcgca tggggttcgg cttcctgtcc
      181 atgaccgccc gcgccggggt cgcgctcgac cccccagtgg atgtctttgt gcaccagagt
      241 aagctgcaca tggaagggtt ccggagcttg aaggagggtg aggcagtgga gttcaccttt
      301 aagaagtcag ccaagggtct ggaatccatc cgtgtcaccg gacctggtgg agtattctgt
      361 attgggagtg agaggcggcc aaaaggaaag agcatgcaga agcgcagatc aaaaggagac
      421 aggtgctaca actgtggagg tctagatcat catgccaagg aatgcaagct gccaccccag
      481 cccaagaagt gccacttctg ccagagcatc agccatatgg tagcctcatg tccgctgaag
      541 gcccagcagg gccctagtgc acagggaaag ccaacctact ttcgagagga agaagaagaa
      601 atccacagcc ctaccctgct cccggaggca cagaattagt aatgaactag tccgtaactt
      661 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgagtc ataattatgc
      721 ttgttgcttc t
//
