LOCUS       sequence-276700-        1064 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing the SMAD3 transcription factor 
            ORF with a unique 24-bp barcode. Barcodes facilitate identification 
            of transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1064)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0114
FEATURES             Location/Qualifiers
     source          1..1064
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagctgtg tgagttcgcc ttcaatatga agaaggacga ggtctgcgtg
       61 aatccctacc actaccagag agtagagaca ccagttctac ctcctgtgtt ggtgccacgc
      121 cacacagaga tcccggccga gttcccccca ctggacgact acagccattc catccccgaa
      181 aacactaact tccccgcagg catcgagccc cagagcaata ttccagagac cccaccccct
      241 ggctacctga gtgaagatgg agaaaccagt gaccaccaga tgaaccacag catggacgca
      301 ggttctccaa acctatcccc gaatccgatg tccccagcac ataataactt ggacctgcag
      361 ccagttacct actgcgagcc ggccttctgg tgctccatct cctactacga gctgaaccag
      421 cgcgtcgggg agacattcca cgcctcgcag ccatccatga ctgtggatgg cttcaccgac
      481 ccctccaatt cggagcgctt ctgcctaggg ctgctctcca atgtcaacag gaatgcagca
      541 gtggagctga cacggagaca catcggaaga ggcgtgcggc tctactacat cggaggggag
      601 gtcttcgcag agtgcctcag tgacagcgct atttttgtcc agtctcccaa ctgtaaccag
      661 cgctatggct ggcacccggc caccgtctgc aagatcccac caggatgcaa cctgaagatc
      721 ttcaacaacc aggagttcgc tgccctcctg gcccagtcgg tcaaccaggg ctttgaggct
      781 gtctaccagt tgacccgaat gtgcaccatc cgcatgagct tcgtcaaagg ctggggagcg
      841 gagtacagga gacagactgt gaccagtacc ccctgctgga ttgagctgca cctgaatggg
      901 cctttgcagt ggcttgacaa ggtcctcacc cagatgggct ccccaagcat ccgctgttcc
      961 agtgtgtctt agtaatgaac tagtccgtaa cttgaaagta tttcgatttc ttggctttat
     1021 atatcttgtg gaaaggacga agtgtgccgg aataattgac tacg
//
