LOCUS       sequence-276745-         956 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing the PHOX2A transcription factor
            ORF with a unique 24-bp barcode. Barcodes facilitate identification 
            of transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0144
FEATURES             Location/Qualifiers
     source          1..956
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggactactc ctacctcaat tcgtacgact cgtgcgtggc ggccatggag
       61 gcgtccgcct acggcgactt tggcgcctgc agccagcccg gcggcttcca atacagcccc
      121 ctgcggcccg ctttccccgc ggcagggccg ccctgccccg cgctcggctc ctccaactgc
      181 gcacttggcg ccctacgcga ccaccagccc gcgccctact cggcagtgcc ctacaagttc
      241 ttcccagagc catccggcct gcacgagaag cgcaagcagc ggcgcatccg caccacgttc
      301 accagcgcgc agctcaagga gctggagcgc gttttcgctg agacccacta ccccgacatt
      361 tacacgcgtg aggagctggc gctcaagatc gacctcactg aggctcgcgt gcaggtctgg
      421 ttccagaacc gccgggccaa gttccgcaaa caggagcgcg cggccagcgc caagggcgcg
      481 gcgggcgcgg cgggcgccaa aaagggcgag gcgcgctgct cctccgagga cgacgattcc
      541 aaggagtcca cgtgcagccc cacgcccgat agcaccgcct cgctgccgcc gccgcctgcg
      601 cccggcctgg ccagcccgcg cctgagcccc agcccgctgc ccgtcgcact gggctccggg
      661 ccgggacctg ggccggggcc acagccgctc aagggcgcac tgtgggccgg tgtggcgggc
      721 ggtgggggcg gcgggcctgg cgcgggagcg gccgaactac ttaaggcttg gcagccggcg
      781 gagtccggcc ccgggccctt ctccggggtt ctgtcctcct ttcaccggaa gcccggcccc
      841 gccctgaaga ccaatctctt ctagtaatga actagtccgt aacttgaaag tatttcgatt
      901 tcttggcttt atatatcttg tggaaaggac gaacatatgg tgttagaaga tctgtg
//
