LOCUS       sequence-276747-       10333 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing the EWSR1 transcription factor 
            ORF with a unique 24-bp barcode. Barcodes facilitate identification 
            of transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10333)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10333)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0160
FEATURES             Location/Qualifiers
     source          1..10333
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    6190..6778
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6243..6263)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6661..6672)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6850..7083
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7161..7282
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7198..7217)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7252..7271
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7322..7457
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7477..7496)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7478..7496)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7506..7523)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7506..7522)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7515..7537)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7664..8119
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7751..7770)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7961..7982
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8145..8249
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8250..8318,8319..9110)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8468..8487)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9281..9869
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9770..9789
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10023..10040
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10157..10178
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10193..10198,10199..10216,10217..10223)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10231..10247
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10236..10258
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10255..10271
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10255..10271
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10290..10310
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10292..10310
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggcg tccacggatt acagtaccta
     4141 tagccaagct gcagcgcagc agggctacag tgcttacacc gcccagccca ctcaaggata
     4201 tgcacagacc acccaggcat atgggcaaca aagctatgga acctatggac agcccactga
     4261 tgtcagctat acccaggctc agaccactgc aacctatggg cagaccgcct atgcaacttc
     4321 ttatggacag cctcccactg gttatactac tccaactgcc ccccaggcat acagccagcc
     4381 tgtccagggg tatggcactg gtgcttatga taccaccact gctacagtca ccaccaccca
     4441 ggcctcctat gcagctcagt ctgcatatgg cactcagcct gcttatccag cctatgggca
     4501 gcagccagca gccactgcac ctacaagacc gcaggatgga aacaagccca ctgagactag
     4561 tcaacctcaa tctagcacag ggggttacaa ccagcccagc ctaggatatg gacagagtaa
     4621 ctacagttat ccccaggtac ctgggagcta ccccatgcag ccagtcactg cacctccatc
     4681 ctaccctcct accagctatt cctctacaca gccgactagt tatgatcaga gcagttactc
     4741 tcagcagaac acctatgggc aaccgagcag ctatggacag cagagtagct atggtcaaca
     4801 aagcagctat gggcagcagc ctcccactag ttacccaccc caaactggat cctacagcca
     4861 agctccaagt caatatagcc aacagagcag cagctacggg cagcagagtt cattccgaca
     4921 ggaccacccc agtagcatgg gtgtttatgg gcaggagtct ggaggatttt ccggaccagg
     4981 agagaaccgg agcatgagtg gccctgataa ccggggcagg ggaagagggg gatttgatcg
     5041 tggaggcatg agcagaggtg ggcggggagg aggacgcggt ggaatgggcg ctggagagcg
     5101 aggtggcttc aataagcctg gtggacccat ggatgaagga ccagatcttg atctaggccc
     5161 acctgtagat ccagatgaag actctgacaa cagtgcaatt tatgtacaag gattaaatga
     5221 cagtgtgact ctagatgatc tggcagactt ctttaagcag tgtggggttg ttaagatgaa
     5281 caagagaact gggcaaccca tgatccacat ctacctggac aaggaaacag gaaagcccaa
     5341 aggcgatgcc acagtgtcct atgaagaccc acccactgcc aaggctgccg tggaatggtt
     5401 tgatgggaaa gattttcaag ggagcaaact taaagtctcc cttgctcgga agaagcctcc
     5461 aatgaacagt atgcggggtg gtctgccacc ccgtgagggc agaggcatgc caccaccact
     5521 ccgtggaggt ccaggaggcc caggaggtcc tgggggaccc atgggtcgca tgggaggccg
     5581 tggaggagat agaggaggct tccctccaag aggaccccgg ggttcccgag ggaacccctc
     5641 tggaggagga aacgtccagc accgagctgg agactggcag tgtcccaatc cgggttgtgg
     5701 aaaccagaac ttcgcctgga gaacagagtg caaccagtgt aaggccccaa agcctgaagg
     5761 cttcctcccg ccaccctttc cgcccccggg tggtgatcgt ggcagaggtg gccctggtgg
     5821 catgcgggga ggaagaggtg gcctcatgga tcgtggtggt cccggtggaa tgttcagagg
     5881 tggccgtggt ggagacagag gtggcttccg tggtggccgg ggcatggacc gaggtggctt
     5941 tggtggagga agacgaggtg gccctggggg gccccctgga cctttgatgg aacagatggg
     6001 aggaagaaga ggaggacgtg gaggacctgg aaaaatggat aaaggcgagc accgtcagga
     6061 gcgcagagat cggccctact agtaatgaac tagtccgtaa cttgaaagta tttcgatttc
     6121 ttggctttat atatcttgtg gaaaggacga aatgcctccg tagcgatgta catgacgcgt
     6181 taagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     6241 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     6301 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     6361 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     6421 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     6481 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     6541 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
     6601 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     6661 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     6721 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcgt
     6781 cgactttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     6841 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta
     6901 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     6961 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     7021 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     7081 gcagtacgta tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     7141 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
     7201 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     7261 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
     7321 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     7381 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     7441 aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg
     7501 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     7561 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     7621 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc
     7681 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     7741 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     7801 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     7861 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     7921 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     7981 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     8041 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     8101 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     8161 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     8221 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     8281 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     8341 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     8401 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     8461 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     8521 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     8581 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     8641 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     8701 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     8761 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     8821 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     8881 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     8941 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     9001 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     9061 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     9121 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     9181 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     9241 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     9301 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     9361 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     9421 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     9481 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     9541 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     9601 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     9661 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     9721 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     9781 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     9841 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     9901 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     9961 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
    10021 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
    10081 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
    10141 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
    10201 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
    10261 acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc
    10321 tggagctgca agc
//
