LOCUS       sequence-276748-        2069 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing the EWSR1 transcription factor 
            ORF with a unique 24-bp barcode. Barcodes facilitate identification 
            of transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0160
FEATURES             Location/Qualifiers
     source          1..2069
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcgtccac ggattacagt acctatagcc aagctgcagc gcagcagggc
       61 tacagtgctt acaccgccca gcccactcaa ggatatgcac agaccaccca ggcatatggg
      121 caacaaagct atggaaccta tggacagccc actgatgtca gctataccca ggctcagacc
      181 actgcaacct atgggcagac cgcctatgca acttcttatg gacagcctcc cactggttat
      241 actactccaa ctgcccccca ggcatacagc cagcctgtcc aggggtatgg cactggtgct
      301 tatgatacca ccactgctac agtcaccacc acccaggcct cctatgcagc tcagtctgca
      361 tatggcactc agcctgctta tccagcctat gggcagcagc cagcagccac tgcacctaca
      421 agaccgcagg atggaaacaa gcccactgag actagtcaac ctcaatctag cacagggggt
      481 tacaaccagc ccagcctagg atatggacag agtaactaca gttatcccca ggtacctggg
      541 agctacccca tgcagccagt cactgcacct ccatcctacc ctcctaccag ctattcctct
      601 acacagccga ctagttatga tcagagcagt tactctcagc agaacaccta tgggcaaccg
      661 agcagctatg gacagcagag tagctatggt caacaaagca gctatgggca gcagcctccc
      721 actagttacc caccccaaac tggatcctac agccaagctc caagtcaata tagccaacag
      781 agcagcagct acgggcagca gagttcattc cgacaggacc accccagtag catgggtgtt
      841 tatgggcagg agtctggagg attttccgga ccaggagaga accggagcat gagtggccct
      901 gataaccggg gcaggggaag agggggattt gatcgtggag gcatgagcag aggtgggcgg
      961 ggaggaggac gcggtggaat gggcgctgga gagcgaggtg gcttcaataa gcctggtgga
     1021 cccatggatg aaggaccaga tcttgatcta ggcccacctg tagatccaga tgaagactct
     1081 gacaacagtg caatttatgt acaaggatta aatgacagtg tgactctaga tgatctggca
     1141 gacttcttta agcagtgtgg ggttgttaag atgaacaaga gaactgggca acccatgatc
     1201 cacatctacc tggacaagga aacaggaaag cccaaaggcg atgccacagt gtcctatgaa
     1261 gacccaccca ctgccaaggc tgccgtggaa tggtttgatg ggaaagattt tcaagggagc
     1321 aaacttaaag tctcccttgc tcggaagaag cctccaatga acagtatgcg gggtggtctg
     1381 ccaccccgtg agggcagagg catgccacca ccactccgtg gaggtccagg aggcccagga
     1441 ggtcctgggg gacccatggg tcgcatggga ggccgtggag gagatagagg aggcttccct
     1501 ccaagaggac cccggggttc ccgagggaac ccctctggag gaggaaacgt ccagcaccga
     1561 gctggagact ggcagtgtcc caatccgggt tgtggaaacc agaacttcgc ctggagaaca
     1621 gagtgcaacc agtgtaaggc cccaaagcct gaaggcttcc tcccgccacc ctttccgccc
     1681 ccgggtggtg atcgtggcag aggtggccct ggtggcatgc ggggaggaag aggtggcctc
     1741 atggatcgtg gtggtcccgg tggaatgttc agaggtggcc gtggtggaga cagaggtggc
     1801 ttccgtggtg gccggggcat ggaccgaggt ggctttggtg gaggaagacg aggtggccct
     1861 ggggggcccc ctggaccttt gatggaacag atgggaggaa gaagaggagg acgtggagga
     1921 cctggaaaaa tggataaagg cgagcaccgt caggagcgca gagatcggcc ctactagtaa
     1981 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     2041 gacgaaatgc ctccgtagcg atgtacatg
//
