LOCUS       sequence-276766-        1748 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing the RELA transcription factor 
            ORF with a unique 24-bp barcode. Barcodes facilitate identification 
            of transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1748)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0176
FEATURES             Location/Qualifiers
     source          1..1748
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1282..1653
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
ORIGIN
        1 accgccacca tggacgaact gttccccctc atcttcccgg cagagccagc ccaggcctct
       61 ggcccctatg tggagatcat tgagcagccc aagcagcggg gcatgcgctt ccgctacaag
      121 tgcgaggggc gctccgcggg cagcatccca ggcgagagga gcacagatac caccaagacc
      181 caccccacca tcaagatcaa tggctacaca ggaccaggga cagtgcgcat ctccctggtc
      241 accaaggacc ctcctcaccg gcctcacccc cacgagcttg taggaaagga ctgccgggat
      301 ggcttctatg aggctgagct ctgcccggac cgctgcatcc acagtttcca gaacctggga
      361 atccagtgtg tgaagaagcg ggacctggag caggctatca gtcagcgcat ccagaccaac
      421 aacaacccct tccaagaaga gcagcgtggg gactacgacc tgaatgctgt gcggctctgc
      481 ttccaggtga cagtgcggga cccatcaggc aggcccctcc gcctgccgcc tgtcctttct
      541 catcccatct ttgacaatcg tgcccccaac actgccgagc tcaagatctg ccgagtgaac
      601 cgaaactctg gcagctgcct cggtggggat gagatcttcc tactgtgtga caaggtgcag
      661 aaagaggaca ttgaggtgta tttcacggga ccaggctggg aggcccgagg ctccttttcg
      721 caagctgatg tgcaccgaca agtggccatt gtgttccgga cccctcccta cgcagacccc
      781 agcctgcagg ctcctgtgcg tgtctccatg cagctgcggc ggccttccga ccgggagctc
      841 agtgagccca tggaattcca gtacctgcca gatacagacg atcgtcaccg gattgaggag
      901 aaacgtaaaa ggacatatga gaccttcaag agcatcatga agaagagtcc tttcagcgga
      961 cccaccgacc cccggcctcc acctcgacgc attgctgtgc cttcccgcag ctcagcttct
     1021 gtccccaagc cagcacccca gccctatccc tttacgtcat ccctgagcac catcaactat
     1081 gatgagtttc ccaccatggt gtttccttct gggcagatca gccaggcctc ggccttggcc
     1141 ccggcccctc cccaagtcct gccccaggct ccagcccctg cccctgctcc agccatggta
     1201 tcagctctgg cccaggcccc agcccctgtc ccagtcctag ccccaggccc tcctcaggct
     1261 gtggccccac ctgcccccaa gcccacccag gctggggaag gaacgctgtc agaggccctg
     1321 ctgcagctgc agtttgatga tgaagacctg ggggccttgc ttggcaacag cacagaccca
     1381 gctgtgttca cagacctggc atccgtcgac aactccgagt ttcagcagct gctgaaccag
     1441 ggcatacctg tggcccccca cacaactgag cccatgctga tggagtaccc tgaggctata
     1501 actcgcctag tgacaggggc ccagaggccc cccgacccag ctcctgctcc actgggggcc
     1561 ccggggctcc ccaatggcct cctttcagga gatgaagact tctcctccat tgcggacatg
     1621 gacttctcag ccctgctgag tcagatcagc tcctagtaat gaactagtcc gtaacttgaa
     1681 agtatttcga tttcttggct ttatatatct tgtggaaagg acgacgcgag gacgggagtc
     1741 ttcagata
//
