LOCUS       sequence-276789-       10537 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing the RFX2 transcription factor 
            ORF with a unique 24-bp barcode. Barcodes facilitate identification 
            of transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10537)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10537)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0199
FEATURES             Location/Qualifiers
     source          1..10537
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    6394..6982
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6447..6467)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6865..6876)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7054..7287
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7365..7486
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7402..7421)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7456..7475
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7526..7661
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7681..7700)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7682..7700)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7710..7727)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7710..7726)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7719..7741)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7868..8323
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7955..7974)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8165..8186
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8349..8453
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8454..8522,8523..9314)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8672..8691)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9485..10073
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9974..9993
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10227..10244
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10361..10382
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10397..10402,10403..10420,10421..10427)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10435..10451
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10440..10462
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10459..10475
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10459..10475
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10494..10514
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10496..10514
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgcag aattccgagg gtggagcgga
     4141 ttcgccagcg tccgtggctc tgcgtccctc ggcggcagcc ccgcctgtgc cagcctcccc
     4201 gcagagggtg ttggtccagg cagccagctc caatcccaaa ggggcccaga tgcagccgat
     4261 ctccctcccc agagttcagc aggtacccca gcaggtgcag ccggtgcagc acgtgtatcc
     4321 tgcccaggtg cagtacgtgg aagggggaga cgccgtctac accaatggag ccatacgaac
     4381 agcctacacc tacaaccccg agcctcagat gtacgccccc agcagcacgg cttcttactt
     4441 cgaggcccca ggcggtgccc aggtgaccgt ggcagcctcg tccccgccag cggtcccctc
     4501 ccacagcatg gtgggcatca ccatggatgt cggggggagc cccatcgtct ccagcgcggg
     4561 agcctatctc atccacgggg ggatggacag caccagacac tccctggccc acacctcccg
     4621 ctcatcgccc gccacgcttg aaatggcgat tgaaaacctc caaaaaagcg aaggaatcac
     4681 atcacacaaa agcggtttac tcaacagcca tctccagtgg ctgttggata attatgaaac
     4741 agcggaaggt gtgagtctcc ccagaagttc tctttacaac cactaccttc ggcactgcca
     4801 ggagcacaag ctagacccag tgaacgccgc ctccttcggg aaactgatcc gttctgtgtt
     4861 tatggggctg agaacgcggc ggctgggcac caggggcaac tcgaagtacc attactatgg
     4921 gattcgtctg aagccggact caccactgaa ccggctgcag gaggacacgc agtacatggc
     4981 catgcggcag cagcccatgc accagaagcc caggtaccgg ccagcccaga agacggacag
     5041 cctcggggac agcggctccc acagcggcct gcacagcact ccggaacaga ccatggccgt
     5101 gcagagccag caccaccagc agtacataga tgtctcccac gtcttccccg agttcccagc
     5161 gcccgacctg ggcagcttcc tgctgcagga cggcgtcaca ctgcacgacg tcaaggccct
     5221 gcagctggtg tacagacggc actgcgaggc aactgtagat gtggtgatga acctccagtt
     5281 ccactacatc gagaagctgt ggctctcctt ctggaactct aaggcctcct ccagcgacgg
     5341 ccccacctct cttcctgcca gtgacgaaga ccccgagggc gccgtcctgc ccaaggacaa
     5401 gcttatctcc ctgtgtcagt gcgaccccat cctcaggtgg atgaggagct gcgaccacat
     5461 cctctaccag gcgctggtgg agattctcat ccccgacgtg ctgaggccgg tccccagtac
     5521 cttgacacag gccatccgta actttgccaa gagcttggaa ggctggttga caaatgccat
     5581 gagtgacttc ccacaacagg tcatccagac caaggtgggc gtcgtcagtg ccttcgccca
     5641 gacgctgcgg cgctacacgt ccctcaacca cctggcgcag gcggcccggg cggtgctgca
     5701 gaacacgtcc cagatcaacc agatgctcag cgacctcaac cgcgtggact ttgccaacgt
     5761 gcaggagcag gcctcgtggg tgtgccagtg cgaggagagt gtggtgcagc ggctggagca
     5821 ggatttcaag ctgaccctgc agcagcagag ctccctggac cagtgggcca gctggctgga
     5881 cagtgtggtc acccaggtcc tgaagcagca tgccggcagc cccagcttcc ccaaggccgc
     5941 ccggcagttc ttgctgaaat ggtcctttta cagctccatg gtgatccggg acctgaccct
     6001 gcgcagcgct gccagcttcg gctccttcca cctcatccgc ctgctctacg acgagtacat
     6061 gttctacctg gtggagcacc gcgtcgcgga ggccaccgga gagacgccga tcgctgtgat
     6121 gggagagttc aacgatctcg cctctctgtc gctgacgctg ctcgacaaag atgacatggg
     6181 cgatgagcag cgtggcagcg aggcgggccc agacgcccgc agcctgggtg agcccctggt
     6241 aaagcgggag cgcagtgacc ccaaccactc cctgcagggc atctagtaat gaactagtcc
     6301 gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg acgatgtcag
     6361 caatcacagc aggccatgac gcgttaagtc gacaatcaac ctctggatta caaaatttgt
     6421 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     6481 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     6541 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     6601 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     6661 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     6721 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     6781 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     6841 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     6901 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     6961 tccctttggg ccgcctcccc gcgtcgactt taagaccaat gacttacaag gcagctgtag
     7021 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     7081 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     7141 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     7201 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     7261 tttagtcagt gtggaaaatc tctagcagta cgtatagtag ttcatgtcat cttattattc
     7321 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag
     7381 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     7441 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct
     7501 agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     7561 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     7621 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg
     7681 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg
     7741 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     7801 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     7861 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     7921 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     7981 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     8041 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     8101 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     8161 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     8221 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     8281 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg
     8341 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     8401 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     8461 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     8521 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     8581 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     8641 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     8701 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     8761 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     8821 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     8881 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     8941 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     9001 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     9061 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     9121 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     9181 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     9241 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     9301 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     9361 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     9421 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     9481 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     9541 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     9601 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     9661 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     9721 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     9781 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     9841 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     9901 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     9961 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
    10021 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
    10081 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
    10141 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
    10201 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
    10261 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
    10321 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
    10381 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
    10441 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta
    10501 accctcacta aagggaacaa aagctggagc tgcaagc
//
