LOCUS       sequence-276883-        1970 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing the MSLN transcription factor 
            ORF with a unique 24-bp barcode. Barcodes facilitate identification 
            of transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0320
FEATURES             Location/Qualifiers
     source          1..1970
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggccttgcc aacggctcga cccctgttgg ggtcctgtgg gacccccgcc
       61 ctcggcagcc tcctgttcct gctcttcagc ctcggatggg tgcagccctc gaggaccctg
      121 gctggagaga cagggcagga ggctgcgccc ctggacggag tcctggccaa cccacctaac
      181 atttccagcc tctcccctcg ccaactcctt ggcttcccgt gtgcggaggt gtccggcctg
      241 agcacggagc gtgtccggga gctggctgtg gccttggcac agaagaatgt caagctctca
      301 acagagcagc tgcgctgtct ggctcaccgg ctctctgagc cccccgagga cctggacgcc
      361 ctcccattgg acctgctgct attcctcaac ccagatgcgt tctcggggcc ccaggcctgc
      421 acccgtttct tctcccgcat cacgaaggcc aatgtggacc tgctcccgag gggggctccc
      481 gagcgacagc ggctgctgcc tgcggctctg gcctgctggg gtgtgcgggg gtctctgctg
      541 agcgaggctg atgtgcgggc tctgggaggc ctggcttgcg acctgcctgg gcgctttgtg
      601 gccgagtcgg ccgaagtgct gctaccccgg ctggtgagct gcccgggacc cctggaccag
      661 gaccagcagg aggcagccag ggcggctctg cagggcgggg gaccccccta cggccccccg
      721 tcgacatggt ctgtctccac gatggacgct ctgcggggcc tgctgcccgt gctgggccag
      781 cccatcatcc gcagcatccc gcagggcatc gtggccgcgt ggcggcaacg ctcctctcgg
      841 gacccatcct ggcggcagcc tgaacggacc atcctccggc cgcggttccg gcgggaagtg
      901 gagaagacag cctgtccttc aggcaagaag gcccgcgaga tagacgagag cctcatcttc
      961 tacaagaagt gggagctgga agcctgcgtg gatgcggccc tgctggccac ccagatggac
     1021 cgcgtgaacg ccatcccctt cacctacgag cagctggacg tcctaaagca taaactggat
     1081 gagctctacc cacaaggtta ccccgagtct gtgatccagc acctgggcta cctcttcctc
     1141 aagatgagcc ctgaggacat tcgcaagtgg aatgtgacgt ccctggagac cctgaaggct
     1201 ttgcttgaag tcaacaaagg gcacgaaatg agtcctcagg tggccaccct gatcgaccgc
     1261 tttgtgaagg gaaggggcca gctagacaaa gacaccctag acaccctgac cgccttctac
     1321 cctgggtacc tgtgctccct cagccccgag gagctgagct ccgtgccccc cagcagcatc
     1381 tgggcggtca ggccccagga cctggacacg tgtgacccaa ggcagctgga cgtcctctat
     1441 cccaaggccc gccttgcttt ccagaacatg aacgggtccg aatacttcgt gaagatccag
     1501 tccttcctgg gtggggcccc cacggaggat ttgaaggcgc tcagtcagca gaatgtgagc
     1561 atggacttgg ccacgttcat gaagctgcgg acggatgcgg tgctgccgtt gactgtggct
     1621 gaggtgcaga aacttctggg accccacgtg gagggcctga aggcggagga gcggcaccgc
     1681 ccggtgcggg actggatcct acggcagcgg caggacgacc tggacacgct ggggctgggg
     1741 ctacagggcg gcatccccaa cggctacctg gtcctagacc tcagcatgca agaggccctc
     1801 tcggggacgc cctgcctcct aggacctgga cctgttctca ccgtcctggc actgctccta
     1861 gcctccaccc tggcctagta atgaactagt ccgtaacttg aaagtatttc gatttcttgg
     1921 ctttatatat cttgtggaaa ggacgagtgt tctatccgtc tatcttcact
//
