LOCUS       sequence-27692-e         754 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Cavrois M, De Noronha C, Greene WC
  TITLE     A sensitive and specific enzyme-based assay detecting HIV-1 virion 
            fusion in primary T lymphocytes.
  JOURNAL   Nat Biotechnol. 2002 Nov . 20(11):1151-4.
  PUBMED    12355096
REFERENCE   2  (bases 1 to 754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2002 Nov . 20(11):1151-4."
COMMENT     Sequence Label: pCMV4-BlaM-Vpr
FEATURES             Location/Qualifiers
     source          1..754
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             209..587
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     complement(243..262)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gagctcgttt agtgaaccgt cagaattgtt tttattttta attttctttc aaatacttcc
       61 atcgaattca gatctggtac cacgcgtggg atggacccag aaacgctggt gaaagtaaaa
      121 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
      181 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
      241 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
      301 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
      361 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
      421 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
      481 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
      541 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
      601 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
      661 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
      721 tctggagccg gtgagcgtgg gtctcgcggt atca
//
