LOCUS       sequence-27693-e         647 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Guang W, Kim KC, Lillehoj EP
  TITLE     MUC1 mucin interacts with calcium-modulating cyclophilin ligand.
  JOURNAL   Int J Biochem Cell Biol. 2009 Jun . 41(6):1354-60.
  PUBMED    19135167
REFERENCE   2  (bases 1 to 647)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Int J 
            Biochem Cell Biol. 2009 Jun . 41(6):1354-60."
COMMENT     Sequence Label: pcDNA-HA-CAML
FEATURES             Location/Qualifiers
     source          1..647
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     21..40
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        21..39
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      80..89
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             92..118
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gcttactggc ttatcgaaat taatacgact cactataggg agacccaagc tggctagcgc
       61 taccggactc agatctcgag ccaccatgga gtacccatac gacgtaccag attacgctca
      121 tatggagtcg atggccgtcg ctaccgacgg cggggagagg ccgggggtcc cagcgggctc
      181 aggtctgtcg gcttcccagc gtcgggcgga gctgcgtcgg agaaagctgc tcatgaactc
      241 ggaacagcgc atcaaccgga tcatgggctt tcacaggccc gggagcggcg cggaagaaga
      301 aagtcaaaca aaatcaaagc agcaggacag tgataaactg aactccctca gcgttccttc
      361 cgtttcaaag cgagtagtgc tgggtgattc agtcagtaca ggaacaactg accagcaggg
      421 tggtgtggcc gaggtaaagg ggacccaact gggagacaaa ttggactcgt tcattaaacc
      481 acctgagtgc agtagtgatg tcaaccttga gctccggcag cggaacagag gggacctgac
      541 agcggactcg gtccagaggg gttcccgcca tggcctagag cagtaccttt ccagattcga
      601 agaagcaatg aagctaagga aacagctgat tagtgaaaaa cccagtc
//
