LOCUS       sequence-276985-        1334 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing the THRA transcription factor 
            ORF with a unique 24-bp barcode. Barcodes facilitate identification 
            of transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1334)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0451
FEATURES             Location/Qualifiers
     source          1..1334
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaacagaa gccaagcaag gtggagtgtg ggtcagaccc agaggagaac
       61 agtgccaggt caccagatgg aaagcgaaaa agaaagaacg gccaatgttc cctgaaaacc
      121 agcatgtcag ggtatatccc tagttacctg gacaaagacg agcagtgtgt cgtgtgtggg
      181 gacaaggcaa ctggttatca ctaccgctgt atcacttgtg agggctgcaa gggcttcttt
      241 cgccgcacaa tccagaagaa cctccatccc acctattcct gcaaatatga cagctgctgt
      301 gtcattgaca agatcacccg caatcagtgc cagctgtgcc gcttcaagaa gtgcatcgcc
      361 gtgggcatgg ccatggactt ggttctagat gactcgaagc gggtggccaa gcgtaagctg
      421 attgagcaga accgggagcg gcggcggaag gaggagatga tccgatcact gcagcagcga
      481 ccagagccca ctcctgaaga gtgggatctg atccacattg ccacagaggc ccatcgcagc
      541 accaatgccc agggcagcca ttggaaacag aggcggaaat tcctgcccga tgacattggc
      601 cagtcaccca ttgtctccat gccggacgga gacaaggtgg acctggaagc cttcagcgag
      661 tttaccaaga tcatcacccc ggccatcacc cgtgtggtgg actttgccaa aaaactgccc
      721 atgttctccg agctgccttg cgaagaccag atcatcctcc tgaaggggtg ctgcatggag
      781 atcatgtccc tgcgggcggc tgtccgctac gaccctgaga gcgacaccct gacgctgagt
      841 ggggagatgg ctgtcaagcg ggagcagctc aagaatggcg gcctgggcgt agtctccgac
      901 gccatctttg aactgggcaa gtcactctct gcctttaacc tggatgacac ggaagtggct
      961 ctgctgcagg ctgtgctgct aatgtcaaca gaccgctcgg gcctgctgtg tgtggacaag
     1021 atcgagaaga gtcaggaggc gtacctgctg gcgttcgagc actacgtcaa ccaccgcaaa
     1081 cacaacattc cgcacttctg gcccaagctg ctgatgaagg tgactgacct ccgcatgatc
     1141 ggggcctgcc acgccagccg cttcctccac atgaaagtcg agtgccccac cgaactcttc
     1201 cccccactct tcctcgaggt ctttgaggat caggaagtct agtaatgaac tagtccgtaa
     1261 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga agtaaagatt
     1321 ccctcctaga tctc
//
