LOCUS       sequence-277021-        1175 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing the PAX5 transcription factor 
            ORF with a unique 24-bp barcode. Barcodes facilitate identification 
            of transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1175)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0518
FEATURES             Location/Qualifiers
     source          1..1175
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggatttaga gaaaaattat ccgactcctc ggaccagcag gacaggacat
       61 ggaggagtga atcagcttgg gggggttttt gtgaatggac ggccactccc ggatgtagtc
      121 cgccagagga tagtggaact tgctcatcaa ggtgtcaggc cctgcgacat ctccaggcag
      181 cttcgggtca gccatggttg tgtcagcaaa attcttggca ggtattatga gacaggaagc
      241 atcaagcctg gggtaattgg aggatccaaa ccaaaggtcg ccacacccaa agtggtggaa
      301 aaaatcgctg aatataaacg ccaaaatccc accatgtttg cctgggagat cagggaccgg
      361 ctgctggcag agcgggtgtg tgacaatgac accgtgccta gcgtcagttc catcaacagg
      421 atcatccgga caaaagtaca gcagccaccc aaccaaccag tcccagcttc cagtcacagc
      481 atagtgtcca ctggctccgt gacgcaggtg tcctcggtga gcacggattc ggccggctcg
      541 tcgtactcca tcagcggcat cctgggcatc acgtccccca gcgccgacac caacaagcgc
      601 aagagagacg aaggtattca ggagtctccg gtgccgaacg gccactcgct tccgggcaga
      661 gacttcctcc ggaagcagat gcggggagac ttgttcacac agcagcagct ggaggtgctg
      721 gaccgcgtgt ttgagaggca gcactactca gacatcttca ccaccacaga gcccatcaag
      781 cccgagcaga ccacagagta ttcagccatg gcctcgctgg ctggtgggct ggacgacatg
      841 aaggccaatc tggccagccc cacccctgct gacatcggga gcagtgtgcc aggcccgcag
      901 tcctacccca ttgtgacagg gagtgagttt tccgggagtc cctacagcca ccctcagtat
      961 tcctcgtaca acgactcctg gaggttcccc aacccggggc tgcttggctc cccctactat
     1021 tatagcgctg ccgcccgagg agccgcccca cctgcagccg ccactgccta tgaccgtcac
     1081 tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     1141 ggaaaggacg agttgacctt tcacaacaag gtgat
//
