LOCUS       sequence-277120-        2225 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0684
FEATURES             Location/Qualifiers
     source          1..2225
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1063..1080
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 accgccacca tgcacggagc cgccagagcg ccagccacca gcgtgagtgc cgactgctgc
       61 atcccggccg gcttgcgcct cggaccggtg cctggtacct tcaagctggg caagtacctg
      121 tcagaccgca gggagcccgg gcctaagaaa aaggtgcgca tggtgagagg ggagctggtg
      181 gacgagtcgg ggggctcccc tctggagtgg atagggttaa tccgggcagc cagaaactcc
      241 caggaacaga ctctggaagc tattgcagac ttacccggag gacagatctt ctaccgagca
      301 ttgcgagacg tccagccagg ggaggagctg acagtgtggt attctaactc cttggctcag
      361 tggttcgaca tccccaccac agcgactccg actcacgacg agaaagggga ggagcgctac
      421 atctgctggt actgctggag gacgtttaga taccccaaca gccttaaggc acacctgcgt
      481 ttccactgcg tgttcagcgg cggtggaggc ggcgccttcc tgcaccacga acacgcggct
      541 cgccaaggcg ccgtcccagc ggctgatggc ctcggtctct ccccaaaacc cccggcgccc
      601 gatttcgccg cgccttccca ggcaggaact ttgcgacccc accccctggg cccgccacca
      661 gttcaggcct gcggtgcgcg ggagggcatc aagcgcgagg cctcttccgc gccctcggcc
      721 acctcgccga ccccaggcaa gtgggggcag cccaagaagg gcaaggagca gctggaccgt
      781 gccctggaca tgagcggagc cgcccgagga caagggcact tcctcggcat cgtgggcggc
      841 tcctcggcgg gggtcggcag cctggctttc taccccggcg tgcgctcagc tttcaagccc
      901 gccggcctag cgagggcggc ggcggccgct cacggcgacc cctaccggga ggagagcagc
      961 agcaagcaag gagccggcct cgctttgggc aggctgctgg gcgggggccg ggcgtgcggg
     1021 cgccccggga gcggggagaa ctcggcggcg ggcggcgcgg gtcaccacca tcaccaccac
     1081 gcgcaccacc accaccatcc caagtgcctg ctcgctgggg acccgccgcc gccgccgccg
     1141 cctggcctgc cctgctctgg ggccctgcgc ggcttccctc tgctctccgt ccccccggaa
     1201 gaggcgtccg ccttcaagca cgtggagcgc gccccgcccg cagccgccgc gctgccagga
     1261 gcgcgttatg cgcagctgcc ccctgcgccg gggttgcccc tcgagcgctg cgcgctgccg
     1321 cccctcgacc cgggcggtct caaagcctat ccgggtggtg agtgcagcca cctgcccgcc
     1381 gtcatgccgg cctttacagt ctacaacggg gagctgctct acggctcacc ggccaccacc
     1441 gcttattacc cgctcaaatt gcacttcggc gggctgctga agtatccgga gtccatctcc
     1501 tacttcagcg ggcctgcagc ggccgcccta agccccgccg agctggggtc gctggccagc
     1561 atcgaccgag agatcgccat gcacaatcag cagctgtccg agatggctgc cgggaagggt
     1621 cgcggacgcc tggactcggg gacgttgcca ccggccgtcg cggcggcggg aggcaccggg
     1681 ggcggcggca gcggaggcag cggcgcaggt aagcccaaga ccggccacct gtgcctctac
     1741 tgtggcaagc tgtactcgcg caagtatggg ctcaagatcc acatgcggac gcacacgggc
     1801 tacaagccac tcaagtgcaa agtctgtctg cggcccttcg gcgaccccag caatctcaac
     1861 aagcacatcc ggctgcacgc cgagggcaat acgccctacc gctgcgagtt ctgcggcaag
     1921 gtacttgtgc gccgccggga cctggagcga catgtcaagt cccgccaccc tggccagagt
     1981 ctgctcgcca aagcgggcga cggcccgggt gccgagcccg gctatccccc ggagcctggg
     2041 gatcccaaga gcgacgacag tgacgtggac gtctgcttca cagacgacca gagcgacccc
     2101 gaggttgggg gcggcgggga gcgcgacttg tagtaatgaa ctagtccgta acttgaaagt
     2161 atttcgattt cttggcttta tatatcttgt ggaaaggacg atccggcatt agctaatatt
     2221 cccac
//
