LOCUS       sequence-277144-        2711 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0709
FEATURES             Location/Qualifiers
     source          1..2711
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagcccga gactgcgctg tggggcccgg atctgcaggg tccggaacag
       61 agccccaacg atgctcacag aggtgccgag agtgaaaacg aagaggagag ccctcggcag
      121 gaaagttctg gggaggagat catcatggga gacccggctc agagtccaga atccaaggac
      181 tcaacagaga tgtccctgga gagatcctcc caggacccct ctgtccccca gaacccccca
      241 accccactgg gtcactccaa tcccttggac caccagatcc ccctggaccc cccagccccg
      301 gaggtagtcc ctaccccatc tgactggacc aaggcctgcg aggccagctg gcagtggggc
      361 gctctcacca catggaacag ccccccagtc gtccccgcca acgagcccag cctgcgggag
      421 ctggtgcagg gccgcccggc gggggcggag aagccctaca tctgcaacga gtgcggcaag
      481 agcttcagcc agtggtccaa gctgctgcgg caccagcgca tccacacggg agagcggccc
      541 aacacctgct ccgagtgcgg caagagcttc acgcagagct cgcacctggt gcagcaccag
      601 cgcacgcaca ccggcgagaa gccctacaag tgccccgact gcggcaagtg cttcagctgg
      661 agctccaacc tggtgcagca ccagcgcacg cacacgggag agaagcccta caagtgcacg
      721 gagtgcgaga aagccttcac ccagagcacc aacctcatca agcaccagcg atcccacacc
      781 ggcgagaagc cctacaagtg cggcgagtgc cgccgggctt tctaccgcag ctcggacctc
      841 atccagcacc aggccaccca cacaggcgag aaaccctaca agtgccccga gtgcgggaag
      901 cgcttcggcc agaaccacaa cctcctcaag caccagaaga tccacgcggg cgagaagcca
      961 taccgctgca ccgagtgcgg gaagagcttc atccagagct cggagctgac ccagcaccag
     1021 cgcacgcaca caggcgagaa gccctacgag tgcctagagt gcggcaagag cttcggccac
     1081 agctccaccc tcatcaagca ccagcggact cacctgcgcg aggacccgtt caagtgccca
     1141 gtgtgcggca agaccttcac cctgagcgcc acgttgctgc ggcaccagcg cacgcacacg
     1201 ggcgagcggc cctacaagtg cccagagtgc ggcaagagct tcagcgtcag ctccaacctc
     1261 atcaaccacc agcgcatcca ccgcggcgag cggccctaca tctgcgccga ctgcggcaag
     1321 agcttcatca tgagctccac ccttatccgc caccagcgca tccacaccgg tgagaagccc
     1381 tacaagtgtt ccgactgcgg caagagcttc atccgcagct cccaccttat ccagcaccgc
     1441 cgcacgcaca ccggcgagaa gccctacaag tgccccgagt gcggcaagag cttcagccag
     1501 agctccaacc ttattaccca cgtccgcacg cacatggacg agaacctgtt cgtgtgctcc
     1561 gactgcggga aggccttcct ggaagcccac gagctggagc agcaccgggt gatccatgag
     1621 agggggaaga ccccagcgcg tagggcccag ggcgacagcc tgctggggct cggggacccc
     1681 tccctgctga ccccgccgcc gggagccaag ccgcacaagt gtctcgtgtg cggaaagggc
     1741 ttcaacgacg agggcatctt catgcaacat cagaggatcc acatcggaga aaacccctac
     1801 aaaaatgcag acggcctcat cgcacacgca gcccccaaac ctcctcagtt acgatcccca
     1861 aggctccctt tcagagggaa ttcctacccc ggggctgcgg agggcagagc ggaggccccc
     1921 ggacagcccc ttaagccgcc ggagggtcag gagggcttca gccagaggcg ggggctgctg
     1981 tcctccaaga cctacatctg ctcccactgc ggagagagct tcctggatcg ctctgtgctc
     2041 ctccagcatc agctcaccca cggcaacgaa aagccctttc tctttcctga ttatagaatt
     2101 ggcctagggg aaggcgcagg gcccagcccc ttcttaagtg ggaagccctt taaatgccct
     2161 gaatgcaaac aaagctttgg cctcagctct gagctgctgc tgcaccagaa agtccatgca
     2221 ggcgggaaga gctcccagaa gagtccagag ctggggaaga gctcttccgt cctcctggag
     2281 catctcagga gccccctggg ggccagaccc taccgctgct cagattgcag ggcctccttc
     2341 ctcgaccgcg tggccctcac ccggcaccaa gaaacccaca cccaggaaaa accccccaat
     2401 cccgaggacc cccctccaga ggcagtcacc ctgtccacag atcaggaagg tgagggcgag
     2461 acccctaccc ccacagagag cagcagccat ggggaagggc aaaaccccaa aaccctagtg
     2521 gaagaaaagc cctatctgtg ccccgagtgt ggagccggct tcacagaagt cgcagccctc
     2581 ctgctccata ggagctgcca cccaggtgtc tccctgtagt aatgaactag tccgtaactt
     2641 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgagaa tttccagctc
     2701 ttggaatggc c
//
