LOCUS       sequence-277156-        3008 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0722
FEATURES             Location/Qualifiers
     source          1..3008
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcagaaga tgatccatat ttgggaaggc ctgaacaaat gtttcatttg
       61 gatccttctt tgactcatac aatatttaat ccagaagtat ttcaaccaca gatggcactg
      121 ccaacagatg gcccatacct tcaaatatta gagcaaccta aacagagagg atttcgtttc
      181 cgttatgtat gtgaaggccc atcccatggt ggactacctg gtgcctctag tgaaaagaac
      241 aagaagtctt accctcaggt caaaatctgc aactatgtgg gaccagcaaa ggttattgtt
      301 cagttggtca caaatggaaa aaatatccac ctgcatgccc acagcctggt gggaaaacac
      361 tgtgaggatg ggatctgcac tgtaactgct ggacccaagg acatggtggt cggcttcgca
      421 aacctgggta tacttcatgt gacaaagaaa aaagtatttg aaacactgga agcacgaatg
      481 acagaggcgt gtataagggg ctataatcct ggactcttgg tgcaccctga ccttgcctat
      541 ttgcaagcag aaggtggagg ggaccggcag ctgggagatc gggaaaaaga gctaatccgc
      601 caagcagctc tgcagcagac caaggagatg gacctcagcg tggtgcggct catgtttaca
      661 gcttttcttc cggatagcac tggcagcttc acaaggcgcc tggaacccgt ggtatcagac
      721 gccatctatg acagtaaagc ccccaatgca tccaacttga aaattgtaag aatggacagg
      781 acagctggat gtgtgactgg aggggaggaa atttatcttc tttgtgacaa agttcagaaa
      841 gatgacatcc agattcgatt ttatgaagag gaagaaaatg gtggagtctg ggaaggattt
      901 ggagattttt cccccacaga tgttcataga caatttgcca ttgtcttcaa aactccaaag
      961 tataaagata ttaatattac aaaaccagcc tctgtgtttg tccagcttcg gaggaaatct
     1021 gacttggaaa ctagtgaacc aaaacctttc ctctactatc ctgaaatcaa agataaagaa
     1081 gaagtgcaga ggaaacgtca gaagctcatg cccaattttt cggatagttt cggcggtggt
     1141 agtggtgctg gagctggagg cggaggcatg tttggtagtg gcggtggagg agggggcact
     1201 ggaagtacag gtccagggta tagcttccca cactatggat ttcctactta tggtgggatt
     1261 actttccatc ctggaactac taaatctaat gctgggatga agcatggaac catggacact
     1321 gaatctaaaa aggaccctga aggttgtgac aaaagtgatg acaaaaacac tgtaaacctc
     1381 tttgggaaag ttattgaaac cacagagcaa gatcaggagc ccagcgaggc caccgttggg
     1441 aatggtgagg tcactctaac gtatgcaaca ggaacaaaag aagagagtgc tggagttcag
     1501 gataacctct ttctagagaa ggctatgcag cttgcaaaga ggcatgccaa tgcccttttc
     1561 gactacgcgg tgacaggaga cgtgaagatg ctgctggccg tccagcgcca tctcactgct
     1621 gtgcaggatg agaatgggga cagtgtctta cacttagcaa tcatccacct tcattctcaa
     1681 cttgtgaggg atctactaga agtcacatct ggtttgattt ctgatgacat tatcaacatg
     1741 agaaatgatc tgtaccagac gcccttgcac ttggcagtga tcactaagca ggaagatgtg
     1801 gtggaggatt tgctgagggc tggggccgac ctgagccttc tggaccgctt gggtaactct
     1861 gttttgcacc tagctgccaa agaaggacat gataaagttc tcagtatctt actcaagcac
     1921 aaaaaggcag cactacttct tgaccacccc aacggggacg gtctgaatgc cattcatcta
     1981 gccatgatga gcaatagcct gccatgtttg ctgctgctgg tggccgctgg ggctgacgtc
     2041 aatgctcagg agcagaagtc cgggcgcaca gcactgcacc tggctgtgga gcacgacaac
     2101 atctcattgg caggctgcct gctcctggag ggtgatgccc atgtggacag tactacctac
     2161 gatggaacca cacccctgca tatagcagct gggagagggt ccaccaggct ggcagctctt
     2221 ctcaaagcag caggagcaga tcccctggtg gagaactttg agcctctcta tgacctggat
     2281 gactcttggg aaaatgcagg agaggatgaa ggagttgtgc ctggaaccac gcctctagat
     2341 atggccacca gctggcaggt atttgacata ttaaatggga aaccatatga gccagagttt
     2401 acatctgatg atttactagc acaaggagac atgaaacagc tggctgaaga tgtgaagctg
     2461 cagctgtata agttactaga aattcctgat ccagacaaaa actgggctac tctggcgcag
     2521 aaattaggtc tggggatact taataatgcc ttccggctga gtcctgctcc ttccaaaaca
     2581 cttatggaca actatgaggt ctctgggggt acagtcagag agctggtgga ggccctgaga
     2641 caaatgggct acaccgaagc aattgaagtg atccaggcag cctccagccc agtgaagacc
     2701 acctctcagg cccactcgct gcctctctcg cctgcctcca caaggcagca aatagacgag
     2761 ctccgagaca gtgacagtgt ctgcgacagc ggcgtggaga catccttccg caaactcagc
     2821 tttaccgagt ctctgaccag tggtgcctca ctgctaactc tcaacaaaat gccccatgat
     2881 tatgggcagg aaggacctct agaaggcaaa atttagtaat gaactagtcc gtaacttgaa
     2941 agtatttcga tttcttggct ttatatatct tgtggaaagg acgagtgcgc tttgcttcac
     3001 cggaacgt
//
