LOCUS       sequence-277162-        1880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0732
FEATURES             Location/Qualifiers
     source          1..1880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggatccaaa ctccatcttg ctttctcctc agccccagat ctgctcccac
       61 ctagcagaag cttgtacgga aggcgagaga agctcatccc ctccagagct ggatagagac
      121 tccccgtttc cctggagtca ggtccccagc tccagcccta cagaccccga atggtttggt
      181 gatgagcaca tccaggcaaa gagggccaga gtggagacca ttgtccgagg catgtgtctc
      241 tccccgaatc ctctggtgcc aggcaatgcg caagctgggg tcagcccacg ctgcccaaag
      301 aaggcccgag agaggaagag gaagcagaac cttcccacac cgcaaggcct cctgatgcca
      361 gcccctgcct gggaccaggg caacaggaag gggggccctc gtgtgagaga acaacttcat
      421 ctgctgaagc aacagctaag acatctgcaa gagcacatcc tacaggctgc caagcccagg
      481 gacacagctc aggggccagg aggctgtggc acggggaaag gccctctgag tgcaaagcag
      541 gggaatggct gtgggcctcg cccctgggtt gtggacggtg accaccagca aggtaccagc
      601 aaggacctct ctggggcaga aaaacaccaa gagtctgaga agcccagttt ccttccttct
      661 ggagcaccag cttcactaga gattctgagg aaagagctga ccagggcagt gtcccaggct
      721 gtggactcgg tattacaaaa ggtactattg gatccaccag gccacctgac tcagctgggc
      781 agaagcttcc aggggcaggt ggcagagggt agaagcgagc cctcacctcc tgtgggaggg
      841 gcctgtaaag atccacttgc tttggctgcc ttgcccagga gggtccagct acaagctggg
      901 gtcccagtag gaaatttatc actggccaag cgtctagatt ctcctaggta ccctatccct
      961 ccaagaatga cccccaaacc ctgtcaggat cccccagcaa actttccctt gactgcacct
     1021 tcccacatcc aggaaaatca gattcttagc cagctactgg gtcatagata caacaatggc
     1081 cattggagta gcagtcctcc ccaggactca tcttcccaga ggcacccctc ctcagagcct
     1141 gccctacgac cttggagaac tactaagccg caaccattgg tcctgagcca gcagcagtgt
     1201 cccttgcctt tcacctctgc ccatctggaa agtctacccc ttcttccctc ggtgaagatg
     1261 gaacagagag gcctgcatgc tgtcatggag gcactgcctt tctctttggt ccacatccag
     1321 gagggtctaa accctggtca cttgaagaag gccaaactaa tgtttttctt cacacgatat
     1381 cccagctcca acctcctgaa ggtttatttt cctgatgttc agttcaaccg ctgcattacc
     1441 tcccagatga tcaagtggtt cagcaacttt cgtgagtttt attacatcca aatggaaaaa
     1501 tctgcccggc aagcaatttc agatggtgtc acaaatccca aaatgctggt ggttctccgc
     1561 aattcagaac tttttcaagc tctcaatatg cactacaaca agggaaatga ctttgaggtt
     1621 ccagattgct tcttggaaat tgccagcttg acgttacagg agttcttcag ggctgtctcc
     1681 gcagggagag actcagatcc ttcctggaag aaacccattt ataaaattat ttcgaaactg
     1741 gacagtgaca tcccagagat attcaaatct tccagctatc cccagtagta atgaactagt
     1801 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgacacc
     1861 aacggctcat cattagccca
//
