LOCUS       sequence-27718-e         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Kaelin WG, Pallas DC, DeCaprio JA, Kaye FJ, Livingston DM
  TITLE     Identification of cellular proteins that can interact specifically 
            with the T/E1A-binding region of the retinoblastoma gene product.
  JOURNAL   Cell. 1991 Feb 8. 64(3):521-32.
  PUBMED    1825028
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 1991 
            Feb 8. 64(3):521-32."
COMMENT     Sequence Label: Rb-379-928-Delta-703-737-pGex2T
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(231..253)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(398..416)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        484..588
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cgcttngata ttgaaggatc agatgaagca gatggaagta aacatctccc aggagagtcc
       61 aaatttcagc aaaaactggc agaaatgact tctactcgaa cacgaatgca aaagcagaaa
      121 atgaatgata gcatggatac ctcaaacaag gaagagaaat gaggatcccc gggaattcat
      181 cgtgactgac tgacgatctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
      241 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
      301 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt
      361 agcgatagcg gagtgtataa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta
      421 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
      481 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
      541 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
      601 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
      661 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
      721 atcgaactgg atctcaccag cggtaagatc ttgaganttt cgcccgaaaa cgtttcnatg
      781 atgacacttt taagtctgct atgtgcgcgt attcccgntg cccggcanac acncgtccnc
      841 tcctatccaa tgctgtgatc ccntccnaac tctng
//
