LOCUS       sequence-277180-        1988 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0746
FEATURES             Location/Qualifiers
     source          1..1988
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcggagct gggcgaatta aagcacatgg tgatgagttt ccgggtgtct
       61 gagctccagg tgcttcttgg ctttgctggc cggaacaaga gtggacggaa gcacgagctc
      121 ctggccaagg ctctgcacct cctgaagtcc agctgtgccc ctagtgtcca gatgaagatc
      181 aaagagcttt accgacgacg ctttccccgg aagaccctgg ggccctctga tctctccctt
      241 ctctctttgc cccctggcac ctctcctgta ggctcccctg gtcctctagc tcccattccc
      301 ccaacgctgt tggcccctgg caccctgctg ggccccaagc gtgaggtgga catgcacccc
      361 cctctgcccc agcctgtgca ccctgatgtc accatgaaac cattgccctt ctatgaagtc
      421 tatggggagc tcatccggcc caccaccctt gcatccactt ctagccagcg gtttgaggaa
      481 gcgcacttta cctttgccct cacaccccag caagtgcagc agattcttac atccagagag
      541 gttctgccag gagccaaatg tgattatacc atacaggtgc agctaaggtt ctgtctctgt
      601 gagaccagct gcccccagga agattatttt ccccccaacc tctttgtcaa ggtcaatggg
      661 aaactgtgcc ccctgccggg ttaccttccc ccaaccaaga atggggccga gcccaagagg
      721 cccagccgcc ccatcaacat cacacccctg gctcgactct cagccactgt tcccaacacc
      781 attgtggtca attggtcatc tgagttcgga cggaattact ccttgtctgt gtacctggtg
      841 aggcagttga ctgcaggaac ccttctacaa aaactcagag caaagggtat ccggaaccca
      901 gaccactcgc gggcactgat caaggagaaa ttgactgctg accctgacag tgaggtggcc
      961 actacaagtc tccgggtgtc actcatgtgc ccgctaggga agatgcgcct gactgtccct
     1021 tgtcgtgccc tcacctgcgc ccacctgcag agcttcgatg ctgcccttta tctacagatg
     1081 aatgagaaga agcctacatg gacatgtcct gtgtgtgaca agaaggctcc ctatgaatct
     1141 cttatcattg atggtttatt tatggagatt cttagttcct gttcagattg tgatgagatc
     1201 caattcatgg aagatggatc ctggtgccca atgaaaccca agaaggaggc atctgaggtt
     1261 tgccccccgc cagggtatgg gctggatggc ctccagtaca gcccagtcca ggggggagat
     1321 ccatcagaga ataagaagaa ggtcgaagtt attgacttga caatagaaag ctcatcagat
     1381 gaggaggatc tgccccctac caagaagcac tgttctgtca cctcagctgc catcccggcc
     1441 ctacctggaa gcaaaggagt cctgacatct ggccaccagc catcctcggt gctaaggagc
     1501 cctgctatgg gcacgttggg tggggatttc ctgtccagtc tcccactaca tgagtaccca
     1561 cctgccttcc cactgggagc cgacatccaa ggtttagatt tattttcatt tcttcagaca
     1621 gagagtcagc actatggccc ctctgtcatc acctcactag atgaacagga tgcccttggc
     1681 cacttcttcc agtaccgagg gaccccttct cactttctgg gcccactggc ccccacgctg
     1741 gggagctccc actgcagcgc cactccggcg ccccctcctg gccgtgtcag cagcattgtg
     1801 gcccctgggg gggccttgag ggaggggcat ggaggacccc tgccctcagg tccctctttg
     1861 actggctgtc ggtcagacat catttccctg gactagtaat gaactagtcc gtaacttgaa
     1921 agtatttcga tttcttggct ttatatatct tgtggaaagg acgagataca tgcatatccc
     1981 tcaattag
//
