LOCUS       sequence-277186-        1634 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0748
FEATURES             Location/Qualifiers
     source          1..1634
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcggcgga gctggtggag gccaaaaaca tggtgatgag ttttcgagtc
       61 tccgaccttc agatgctcct gggtttcgtg ggccggagta agagtggact gaagcacgag
      121 ctcgtcacca gggccctcca gctggtgcag tttgactgta gccctgagct gttcaagaag
      181 atcaaggagc tgtacgagac ccgctacgcc aagaagaact cggagcctgc cccacagccg
      241 caccggcccc tggaccccct gaccatgcac tccacctacg accgggccgg cgctgtgccc
      301 aggactccgc tggcaggccc caatattgac taccccgtgc tctacggaaa gtacttaaac
      361 ggactgggac ggttgcccgc caagaccctc aagccagaag tccgcctggt gaagctgccg
      421 ttctttaata tgctggatga gctgctgaag cccaccgaat tagtcccaca gaacaacgag
      481 aagcttcagg agagcccgtg catcttcgca ttgacgccaa gacaggtgga gttgatccgg
      541 aactccaggg aactgcagcc cggagttaaa gccgtgcagg tcgtcctgag aatctgttac
      601 tcagacacca gctgccctca ggaggaccag tacccgccca acatcgctgt gaaggtcaac
      661 cacagctact gctccgtccc gggctactac ccctccaata agcccggggt ggagcccaag
      721 aggccgtgcc gccccatcaa cctcacccac ctcatgtacc tgtcctcggc caccaaccgc
      781 atcactgtca cctgggggaa ctacggcaag agctactcgg tggccctgta cctggtgcgg
      841 cagctgacct catcggagct gctgcagagg ctgaagacca ttggggtaaa gcacccggag
      901 ctgtgcaagg cactggtcaa ggagaagctg cgccttgatc ctgacagcga gatcgccacc
      961 accggtgtgc gggtgtccct catctgtccg ctggtgaaga tgcggctctc cgtgccctgc
     1021 cgggcagaga cctgtgccca cctgcagtgc ttcgacgccg tcttctacct gcagatgaac
     1081 gagaagaagc ccacctggat gtgccccgtg tgcgacaagc cagcccccta cgaccagctc
     1141 atcatcgacg ggctcctctc gaagatcctg agcgagtgtg aggacgccga cgagatcgag
     1201 tacctggtgg acggctcgtg gtgcccgatc cgcgccgaaa aggagcgcag ctgcagcccg
     1261 cagggcgcca tcctcgtgct gggcccctcg gacgccaatg ggctcctgcc cgcccccagc
     1321 gtcaacggga gcggtgccct gggcagcacg ggtggcggcg gcccggtggg cagcatggag
     1381 aatgggaagc cgggcgccga tgtggtggac ctcacgctgg acagctcatc gtcctcggag
     1441 gatgaggagg aggaggaaga ggaggaggaa gacgaggacg aagaggggcc ccggcccaag
     1501 cgccgctgcc ccttccagaa gggcctggtg ccggcctgct agtaatgaac tagtccgtaa
     1561 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga agtcttgaac
     1621 cttctttaag tatt
//
