LOCUS       sequence-277189-        1973 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1973)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0749
FEATURES             Location/Qualifiers
     source          1..1973
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaggctga agagacgatg gaatgccttc aggagttccc tgaacatcat
       61 aaaatgatcc tcgaccgatt gaatgaacag cgagagcagg accggtttac tgacatcacc
      121 ctaattgtcg acggacacca ttttaaggct cacaaggctg ttttggctgc ttgtagtaag
      181 ttcttctaca aattctttca ggagtttacc caagaaccat tggtggagat agaaggtgtt
      241 agtaaaatgg cctttcgcca tttaattgag ttcacatata cagcaaaatt aatgatacaa
      301 ggagaagaag aagccaatga tgtatggaaa gcagcagagt ttctacaaat gctagaagct
      361 atcaaagccc ttgaagtcag gaacaaagaa aactcagctc ccttagagga aaataccaca
      421 ggaaaaaatg aggccaaaaa aaggaagatt gcagaaactt caaatgttat cactgagtca
      481 ttgccatctg cagaatcaga acctgttgaa attgaggtag agattgccga aggcaccatt
      541 gaagtggaag atgaaggcat cgaaacatta gaggaagtgg cttctgccaa gcagtccgta
      601 aagtacatac agagcacagg ttcctctgat gattctgctc tagcactgtt ggcagatatt
      661 accagcaagt accgtcaagg tgacagaaaa gggcagatta aagaagatgg ctgtccatct
      721 gaccccacga gcaaacaggt agaaggtatt gaaattgtgg aacttcagct gtcacatgtg
      781 aaggacttgt tccattgtga gaaatgtaac cgttcattta aattgtttta ccattttaag
      841 gagcacatga aatcacactc cactgagagt ttcaagtgtg aaatatgcaa taaacgatat
      901 cttcgagaga gcgcatggaa acagcaccta aattgttacc accttgaaga aggtggagtc
      961 agtaagaagc aaagaactgg gaaaaaaatt catgtatgtc agtactgtga gaaacagttt
     1021 gaccattttg gacattttaa agaacatctt cgaaaacata caggtgaaaa accttttgaa
     1081 tgtccaaatt gtcatgaacg atttgctaga aatagcactc tgaaatgtca cctcactgca
     1141 tgccaaactg gagtaggggc aaaaaaagga aggaagaagc tctacgaatg ccaggtctgc
     1201 aacagtgtgt ttaacagctg ggaccagttc aaagatcact tggtaataca cactggagat
     1261 aaacccaacc attgtacttt atgtgatttg tggtttatgc aaggaaatga attaaggagg
     1321 catctcagtg atgctcacaa tatttcagag cgtctagtaa cggaagaagt tctttcagta
     1381 gaaacacgtg tgcaaactga acctgtaaca tcaatgacta ttatagaaca agttgggaag
     1441 gtgcatgtgc taccattgct tcaggttcag gtggattcag cacaagtgac tgtggaacaa
     1501 gtccatccag atctgctcca ggacagccag gtgcacgatt cacacatgag tgagcttcca
     1561 gagcaggtcc aagtgagtta tctagaagtg ggccgaattc agactgaaga aggtactgaa
     1621 gtacatgtag aggagctgca tgttgaacgg gtcaatcaaa tgccagtgga agtacaaact
     1681 gaacttctag aagcagattt ggaccacgtg accccagaaa tcatgaacca agaggagaga
     1741 gagtctagcc aagcagatgc tgctgaggct gccagggaag atcacgaaga tgctgaggat
     1801 ttagagacca agccaacagt ggattctgaa gcagaaaagg cagagaatga ggacagaaca
     1861 gctctgccag ttttagaata gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
     1921 tggctttata tatcttgtgg aaaggacgag tgggcagatc actgttgtaa acg
//
