LOCUS       sequence-277204-        1373 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1373)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0762
FEATURES             Location/Qualifiers
     source          1..1373
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcggctgt cgtcctgccc ccaactgccg cctctcagcg agaaggacac
       61 acagagggcg gagagctggt taatgagctc ctgaaaagct ggctaaaggg cttggtgacc
      121 tttgaggatg tggccgtgga gttcacccag gaggagtggg cattgctgga ccctgcccaa
      181 aggacactgt acagggacgt gatgctggag aactgcagga acctggcctc actggggaac
      241 caagttgata aacctaggct gatctcccag ctggagcaag aagataaagt gatgacagaa
      301 gagagaggaa ttctctcagg tacctgtcca gatgtggaga atccatttaa agccaaaggg
      361 ttaactccta agctgcatgt ttttcgaaaa gaacaatcta gaaatatgaa aatggagagg
      421 aatcatcttg gagcaacact caacgaatgt aatcagtgtt ttaaagtctt cagcacaaaa
      481 tcttccctta cacggcacag gaagattcat actggagaaa gaccctatgg ctgcagtgaa
      541 tgtgggaaat cctacagcag tagatcttac cttgctgttc ataagagaat ccacaatggg
      601 gagaaaccct atgaatgcaa tgactgtggg aaaaccttca gcagcagatc ttaccttact
      661 gttcataaga gaatccacaa tggggagaaa ccctacgaat gcagtgactg tgggaaaacc
      721 ttcagcaatt cctcatacct cagaccgcac ttgagaattc acactggaga aaaaccgtac
      781 aaatgtaacc agtgttttcg tgagttccgc actcagtcaa tcttcacaag gcacaagaga
      841 gttcatacgg gggagggtca ttatgtatgt aatcagtgtg gaaaggcttt cggcacgagg
      901 tcatctcttt cttcgcacta tagcattcat acaggggagt acccttacga atgccacgat
      961 tgtgggagaa ccttcaggag gaggtcgaat ctgacacagc acataagaac tcatactgga
     1021 gaaaaaccct acacatgtaa tgagtgtggg aaatccttta ccaatagctt ttctcttaca
     1081 attcacagga gaatacataa tggagagaaa tcctatgagt gcagtgattg tggaaaatcc
     1141 tttaatgttc tctcatccgt taagaaacac atgagaactc acactggaaa aaaaccctat
     1201 gaatgtaatt attgcgggaa atccttcaca agtaactcct acctttctgt gcatacgaga
     1261 atgcataata ggcaaatgta gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
     1321 tggctttata tatcttgtgg aaaggacgac gcgttacatt ataccctcgt acc
//
