LOCUS       sequence-277207-        1394 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1394)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0763
FEATURES             Location/Qualifiers
     source          1..1394
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcggctgt cgtcctgccc ccaactgccg ctctgtcttc cctgttccca
       61 gcctctcagc gagaaggaca cacagagggc ggagagctgg ttaatgagct cctgaaaagc
      121 tggctaaagg gcttggtgac ctttgaggat gtggccgtgg agttcaccca ggaggagtgg
      181 gcattgctgg accctgccca aaggacactg tacagggacg tgatgctgga gaactgcagg
      241 aacctggcct cactggggaa ccaagttgat aaacctaggc tgatctccca gctggagcaa
      301 gaagataaag tgatgacaga agagagagga attctctcag gtacctgtcc agatgtggag
      361 aatccattta aagccaaagg gttaactcct aagctgcatg tttttcgaaa agaacaatct
      421 agaaatatga aaatggagag gaatcatctt ggagcaacac tcaacgaatg taatcagtgt
      481 tttaaagtct tcagcacaaa atcttccctt acacggcaca ggaagattca tactggagaa
      541 agaccctatg gctgcagtga atgtgggaaa tcctacagca gtagatctta ccttgctgtt
      601 cataagagaa tccacaatgg ggagaaaccc tatgaatgca atgactgtgg gaaaaccttc
      661 agcagcagat cttaccttac tgttcataag agaatccaca atggggagaa accctacgaa
      721 tgcagtgact gtgggaaaac cttcagcaat tcctcatacc tcagaccgca cttgagaatt
      781 cacactggag aaaaaccgta caaatgtaac cagtgttttc gtgagttccg cactcagtca
      841 atcttcacaa ggcacaagag agttcatacg ggggagggtc attatgtatg taatcagtgt
      901 ggaaaggctt tcggcacgag gtcatctctt tcttcgcact atagcattca tacaggggag
      961 tacccttacg aatgccacga ttgtgggaga accttcagga ggaggtcgaa tctgacacag
     1021 cacataagaa ctcatactgg agaaaaaccc tacacatgta atgagtgtgg gaaatccttt
     1081 accaatagct tttctcttac aattcacagg agaatacata atggagagaa atcctatgag
     1141 tgcagtgatt gtggaaaatc ctttaatgtt ctctcatccg ttaagaaaca catgagaact
     1201 cacactggaa aaaaacccta tgaatgtaat tattgcggga aatccttcac aagtaactcc
     1261 tacctttctg tgcatacgag aatgcataat aggcaaatgt agtaatgaac tagtccgtaa
     1321 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga ctactcgacc
     1381 agaaacgcgt atgc
//
