LOCUS       sequence-277210-        1370 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1370)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0767
FEATURES             Location/Qualifiers
     source          1..1370
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcggagac gaaggacgca gcgcagatgt tggtgacctt caaggatgtg
       61 gctgtgacct ttacccggga ggagtggaga cagctggacc tggcccagag gaccctgtac
      121 cgagaggtga tgctggagac ctgtgggctt ctggtttcac tagggcatcg ggttcccaaa
      181 ccagagttgg tccacctgct agagcatggg caggagctgt ggatagtgaa gagaggcctc
      241 tcacatgcta cctgtgcagg tgacagagca caagttcata ccagagagcc aaccacttac
      301 ccgccagtct tatctgagcg ggcctttctc cggggaagcc tgactctgga atcttcaacc
      361 tcaagtgatt cgaggttggg gagagctagg gatgaggaag ggcttttgga aatgcagaaa
      421 ggtaaagtga caccggagac agacctccac aaggagaccc atcttgggaa agtgagcctt
      481 gaaggtgaag gtttggggac agatgatggt ctgcactcca gggcattaca agagtggtta
      541 tcagcagatg ttctccatga atgtgactca cagcaaccag ggaaagacgc cttgattcat
      601 gcagggacaa acccctacaa atgcaagcag tgtgggaaag gttttaacag gaagtggtat
      661 ctcgttcgac atcagagggt tcacactgga atgaaaccct atgaatgcaa tgcatgtggg
      721 aaagccttta gccagagctc aactctcatt cggcactacc tcatccacac cggggagaaa
      781 ccatacaagt gccttgagtg tgggaaggcc ttcaaacgca ggtcgtacct catgcagcac
      841 catccaatcc acactggaga gaaaccctat gagtgtagtc aatgtcgaaa ggccttcacc
      901 caccggtcta cttttattcg ccataatagg acccacactg gagaaaagcc ctttgagtgc
      961 aaagaatgtg agaaagcctt tagcaataga gcccacctaa ttcaacatta catcatccac
     1021 actggagaga agccctatga ttgcatggca tgtggaaagg ccttcagatg cagctcagaa
     1081 ctcatacagc accagcggat tcacactggg gagaagccct atgaatgcac ccagtgtggg
     1141 aaagcctttc accggagcac gtacctcatt cagcactctg tcatccacac tggggagatg
     1201 ccctacaagt gtatcgagtg tgggaaggcc ttcaaacgca ggtcacacct tctgcagcac
     1261 caaagggtcc atacatagta atgaactagt ccgtaacttg aaagtatttc gatttcttgg
     1321 ctttatatat cttgtggaaa ggacgagaag acatagctat tgttgagaga
//
