LOCUS       sequence-277222-         779 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0778
FEATURES             Location/Qualifiers
     source          1..779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaagagct cacggctttt gtatccaagt cttttgacca gaaaagcaag
       61 gacggtaacg gcggaggcgg aggcggcgga ggtaagaagg attccattac gtaccgggaa
      121 gttttggaga gcggactggc gcgctcccgg gagctgggga cgtcggattc cagcctccag
      181 gacatcacgg agggcggcgg ccactgcccg gtgcatttgt tcaaggacca cgtagacaat
      241 gacaaggaga aactgaaaga attcggcacc gcgagagtgg cagaagggat ttatgaatgc
      301 aaagagaagc gcgaggacgt gaagtcggag gacgaggacg ggcagaccaa gctgaaacag
      361 aggcgcagcc gcaccaactt cacgctggag cagctgaacg agctcgagcg actcttcgac
      421 gagacccatt accccgacgc cttcatgcgc gaggagctca gccagcgcct ggggctctcc
      481 gaggcgcgcg tgcaggtttg gttccagaac cggagagcca agtgccgcaa acaagagaat
      541 cagatgcata aaggcgtcat cttgggcaca gccaaccacc tagacgcctg ccgagtggca
      601 ccctacgtca acatgggagc cttacggatg cctttccaac agatggagtt ttgctcttgt
      661 cgcccgggct ggagtataat ggcatagtaa tgaactagtc cgtaacttga aagtatttcg
      721 atttcttggc tttatatatc ttgtggaaag gacgacccgc tagccagttc cactcacat
//
