LOCUS       sequence-277225-        1289 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1289)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0779
FEATURES             Location/Qualifiers
     source          1..1289
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggatgaaga acctgaaaga actaagcgat gggaaggagg ctatgaaaga
       61 acatgggaga ttcttaaaga agatgaatct ggatcactta aagctacaat agaagacatt
      121 ctattcaagg caaagagaaa aagagtattt gagcaccatg gacaagttcg acttggaatg
      181 atgcgccacc tttatgtggt agtagatgga tcaagaacaa tggaagacca agatttaaag
      241 cctaatagac tgacgtgtac tttaaagttg ttggaatact ttgtagagga atattttgat
      301 caaaatccta ttagtcagat tggaataatt gtaactaaga gtaaaagagc tgaaaaattg
      361 actgaacttt caggaaaccc aagaaaacat ataacgtctt tgaagaaagc tgtggatatg
      421 acctgccatg gagagccatc tctttataat tccctaagca tagctatgca gactctaaaa
      481 cacatgcctg gacatacaag tcgagaagta ctaatcatct ttagcagcct tacaacttgc
      541 gatccatcta atatttatga tctaatcaag accctaaagg cagctaaaat tagagtatct
      601 gttattggat tgtctgcaga agttcgcgtt tgcactgtac ttgctcgtga aactggtggc
      661 acgtaccatg ttattttaga tgaaagccat tacaaagagt tgctcacaca tcatgttagt
      721 cctcctcctg ctagctcaag ttctgaatgc tcacttattc gtatgggatt tcctcagcac
      781 accattgctt ctttatctga ccaggatgca aaaccctctt tcagcatggc gcatttggat
      841 ggcaatactg agccagggct tacattagga ggctatttct gcccacagtg tcgggcaaag
      901 tactgtgagc tacctgttga gtgtaaaatc tgtggtctta ctttggtgtc tgctccccac
      961 ttggcacggt cttaccatca tttgtttcct ttggatgctt ttcaagaaat tcccctagaa
     1021 gaatataatg gagaaagatt ttgttatgga tgtcaggggg aattgaaaga ccaacatgtt
     1081 tatgtttgtg ctgtgtgcca aaatgttttc tgtgtggact gtgatgtttt tgttcatgat
     1141 tctctacact gttgccctgg ctgtattcat aagattccag ctccttcagg tgtttagtaa
     1201 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     1261 gacgaagcct tcgttaggca tgatgccag
//
