LOCUS       sequence-277228-        1748 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1748)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0780
FEATURES             Location/Qualifiers
     source          1..1748
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcaacctc atctgaagaa gttttgctga ttgtaaagaa agtgcgtcaa
       61 aagaagcagg atggagctct gtacctcatg gcagaaagaa ttgcttgggc acctgaaggc
      121 aaagatagat ttacaatcag ccatatgtat gcagatatta aatgccagaa aattagtcca
      181 gaaggaaaag ctaaaattca gcttcagctg gtcctacatg caggggacac aactaacttc
      241 catttttcca atgaaagcac agcagtgaaa gagcgagatg cagtaaaaga ccttcttcag
      301 cagctgctgc ccaaattcaa gaggaaagca aataaagaac tggaagagaa gaacagaatg
      361 ctgcaagaag atcctgtttt gtttcagctt tataaagacc ttgttgtgag tcaagtgatc
      421 agtgctgagg aattctgggc caatcgttta aatgtgaatg caacagatag ttcttccaca
      481 tccaatcata agcaggatgt tggcatttct gctgcatttc tggctgatgt ccggccccaa
      541 actgatggct gtaacggtct aagatataat ttaacttctg atatcattga gtccatattt
      601 aggacctatc cagcagtaaa aatgaaatat gcagaaaatg ttccccacaa catgacagag
      661 aaggaattct ggacacgttt tttccagtcc cattattttc acagggatcg gctgaataca
      721 gggtcaaagg atctctttgc agaatgtgcc aaaatagatg aaaaaggcct aaaaacaatg
      781 gtttcattag gagtgaaaaa cccactacta gatttaacag ctttggaaga taaaccatta
      841 gatgagggct atggcatttc ctctgtgcca tctgcttcca attctaaatc cataaaagag
      901 aatagtaatg ctgccatcat caagagattt aaccatcaca gtgccatggt cctggcagct
      961 ggactcagaa aacaagaagc acaaaatgaa caaactagtg agcccagcaa catggatgga
     1021 aattccggag atgcagactg ctttcagcca gcagtcaaaa gggcgaaatt acaagagtcc
     1081 attgaatatg aagacttggg gaaaaataat tctgtaaaaa cgattgcact aaacctcaag
     1141 aagtcagata ggtattatca tggtccaact ccaatccagt cactacagta tgcaacaagt
     1201 caggacatta ttaattcttt tcaaagtatt agacaagaaa tggaagctta tacacccaag
     1261 ttaactcagg ttctctcaag tagtgctgcc agtagtacca tcacagcact gtcacctgga
     1321 ggggcactta tgcagggagg aacacagcaa gccataaacc agatggtgcc aaatgatatt
     1381 caatctgaat tgaaacactt atatgtagct gttggagaac ttctacgaca tttctggtcc
     1441 tgctttcctg ttaatacgcc attcctagaa gaaaaggtag tgaaaatgaa aagtaatttg
     1501 gaacgattcc aagttacgaa gctctgtcca ttccaagaaa agattcggag acagtattta
     1561 agcacaaatt tggtaagtca catagaagag atgctccaga cagcctacaa caagctccac
     1621 acatggcagt cacggcgtct gatgaagaaa acgtagtaat gaactagtcc gtaacttgaa
     1681 agtatttcga tttcttggct ttatatatct tgtggaaagg acgacttatg cgagagaagc
     1741 cgcgtacg
//
