LOCUS       sequence-27724-e         794 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Bisgrove DA, Mahmoudi T, Henklein P, Verdin E
  TITLE     Conserved P-TEFb-interacting domain of BRD4 inhibits HIV 
            transcription.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 Aug 21. 104(34):13690-5.
  PUBMED    17690245
REFERENCE   2  (bases 1 to 794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2007 Aug 21. 104(34):13690-5."
COMMENT     Sequence Label: GAL4-BRD4
FEATURES             Location/Qualifiers
     source          1..794
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             24..47
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(721..740)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 gcgggattcc ggggatcgtt atggactaca aagacgatga cgacaagctt atgcatccga
       61 ccaccccctc ctccacagcc aagtcatcca gcgacagctt cgagcagttc cgccgcgccg
      121 ctcgggagaa agaggagcgt gagaaggccc tgaaggctca ggccgagcac gctgagaagg
      181 agaaggagcg gctgcggcag gagcgcatga ggagccgaga ggacgaggat gcgctggagc
      241 aggcccggcg ggcccatgag gaggcacgtc ggcgccagga gcagcagcag cagcagcgcc
      301 aggagcaaca gcagcagcag caacagcaag cagctgcggt ggctgccgcc gccaccccac
      361 aggcccagag ctcccagccc cagtccatgc tggaccagca gagggagttg gcccggaagc
      421 gggagcagga gcgaagacgc cgggaagcca tggcagctac cattgacatg aatttccaga
      481 gtgatctatt gtcaatattt gaagaaaatc ttttctgagc gcacctaggt ggatccgtcg
      541 acggtacccc ggggaattcg agctctagat aagtaatgat cataatcagc catatcacat
      601 tgntaaaggt tttacttgtt ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
      661 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
      721 gcaatagcat cacaaatttc acaataagca ttttttcatg cattttattg tggttgtcca
      781 actctcatgt tctt
//
