LOCUS       sequence-277273-        3746 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3746)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0807
FEATURES             Location/Qualifiers
     source          1..3746
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcggtgcg gaagaaggac ggcggcccca acgtgaagta ctacgaggcc
       61 gcggacaccg tgacccagtt cgacaacgtg cggctgtggc tcggcaagaa ctacaagaag
      121 tatatacaag ctgaaccacc caccaacaag tccctgtcta gcctggttgt acagttgcta
      181 caatttcagg aagaagtttt tggcaaacat gtcagcaatg caccgctcac taaactgccg
      241 atcaaatgtt tcctagattt caaagcggga ggctccttgt gccacattct tgcagctgcc
      301 tacaaattca agagtgacca gggatggcgg cgttacgatt tccagaatcc atcacgcatg
      361 gaccgcaatg tggaaatgtt tatgaccatt gagaagtcct tggtgcagaa taattgcctg
      421 tctcgaccta acatttttct gtgcccagaa attgagccca aactactagg gaaattaaag
      481 gacattatca agagacacca gggaacagtc actgaggata agaacaatgc ctcccatgtt
      541 gtgtatcctg tcccggggaa tctagaagaa gaggaatggg tacgaccagt catgaagagg
      601 gataagcagg ttcttctgca ctggggctac tatcctgaca gttacgacac gtggatccca
      661 gcgagtgaaa ttgaggcatc tgtggaagat gctccaactc ctgagaaacc taggaaggtt
      721 catgcaaagt ggatcctgga caccgacacc ttcaatgaat ggatgaatga ggaagactat
      781 gaagtaaatg atgacaaaaa ccctgtctcc cgccgaaaga agatttcagc caagacactg
      841 acagatgagg tgaacagccc agattcagat cgacgggaca agaagggggg aaactataag
      901 aagaggaagc gctccccctc tccttcacca accccagaag caaagaagaa aaatgctaag
      961 aaaggtccct caacacctta cactaagtca aagcgtggcc acagagaaga ggagcaagaa
     1021 gacctgacaa aggacatgga cgagccctca ccagtcccca atgtagaaga ggtgacactt
     1081 cccaaaacag tcaacacaaa gaaagactca gagtcggccc cagtcaaagg cggcaccatg
     1141 accgacctgg atgaacagga agatgaaagc atggagacga cgggcaagga tgaggatgag
     1201 aacagtacgg ggaacaaggg agagcagacc aagaatccag acctgcatga ggacaatgtg
     1261 actgaacaga cccaccacat catcattccc agctacgctg cctggtttga ctacaatagt
     1321 gttcatgcca ttgagcggag ggctctcccc gagttcttca acggcaagaa caagtccaag
     1381 actccagaga tctacctggc ctatcgaaac tttatgattg acacttaccg actgaacccc
     1441 caagagtatc ttacctctac cgcctgccgc cgaaacctag cgggtgatgt ctgtgccatc
     1501 atgagggtcc atgccttcct agaacagtgg ggtcttatta actaccaggt ggatgctgag
     1561 agtcgaccaa ccccaatggg gcctccgcct acctctcact tccatgtctt ggctgacaca
     1621 ccatcagggc tggtgcctct gcagcccaag acacctcagc agacctctgc ttcccaacaa
     1681 atgctcaact ttcctgacaa aggcaaagag aaaccaacag acatgcaaaa ctttgggctg
     1741 cgcacagaca tgtacacaaa aaagaatgtt ccctccaaga gcaaggctgc agccagtgcc
     1801 actcgtgagt ggacagaaca ggaaaccctg cttctcctgg aggcactgga aatgtacaaa
     1861 gatgactgga acaaagtgtc cgagcatgtg ggaagccgca cacaggacga gtgcatcttg
     1921 cattttcttc gtcttcccat tgaagaccca tacctggagg actcagaggc ctccctaggc
     1981 cccctggcct accaacccat ccccttcagt cagtcgggca accctgttat gagcactgtt
     2041 gccttcctgg cctctgtcgt cgatccccga gtcgcctctg ctgctgcaaa gtcagcccta
     2101 gaggagttct ccaaaatgaa ggaagaggta cccacggcct tggtggaggc ccatgttcga
     2161 aaagtggaag aagcagccaa agtaacaggc aaggcggacc ctgccttcgg tctggaaagc
     2221 agtggcattg caggaaccac ctctgatgag cctgagcgga ttgaggagag cgggaatgac
     2281 gaggctcggg tggaaggcca ggccacagat gagaagaagg agcccaagga accccgagaa
     2341 ggagggggtg ctatagagga ggaagcaaaa gagaaaacca gcgaggctcc caagaaggat
     2401 gaggagaaag ggaaagaagg cgacagtgag aaggagtccg agaagagtga tggagaccca
     2461 atagtcgatc ctgagaagga gaaggagcca aaggaagggc aggaggaagt gctgaaggaa
     2521 gtggtggagt ctgaggggga aaggaagaca aaggtggagc gggacattgg cgagggcaac
     2581 ctctccaccg ctgctgccgc cgccctggcc gccgccgcag tgaaagctaa gcacttggct
     2641 gctgttgagg aaaggaagat caaatctttg gtggccctgc tggtggagac ccagatgaaa
     2701 aagttggaga tcaaacttcg gcactttgag gagctggaga ctatcatgga ccgggagcga
     2761 gaagcactgg agtatcagag gcagcagctc ctggccgaca gacaagcctt ccacatggag
     2821 cagctgaagt atgcggagat gagggctcgg cagcagcact tccaacagat gcaccaacag
     2881 cagcagcagc caccaccagc cctgccccca ggctcccagc ctatcccccc aacaggggct
     2941 gctgggccac ccgcagtcca tggcttggct gtggctccag cctctgtagt ccctgctcct
     3001 gctggcagtg gggcccctcc aggaagtttg ggcccttctg aacagattgg gcaggcaggg
     3061 tcaactgcag ggccacagca gcagcaacca gctggagccc cccagcctgg ggcagtccca
     3121 ccaggggttc ccccccctgg accccatggc ccctcaccgt tccccaacca acaaactcct
     3181 ccctcaatga tgccaggggc agtgccaggc agcgggcacc caggcgtggc gggtaatgct
     3241 cctttgggtt tgccttttgg catgccgcct cctcctcctc ctcctgctcc atccatcatc
     3301 ccatttggta gtctagctga ctccatcagt attaacctcc ccgctcctcc taacctgcat
     3361 gggcatcacc accatctccc gttcgccccg ggcactctcc ccccacctaa cctgcctgtg
     3421 tccatggcga accctctaca tcctaacctg ccggcgacca ccaccatgcc atcttccttg
     3481 cctctcgggc cggggctcgg atccgccgca gcccaaagcc ctgccattgt ggcagctgtt
     3541 cagggcaacc tcctgcccag tgccagccca ctgccagacc caggcacccc cctgcctcca
     3601 gaccccacag ccccgagccc aggcacggtc acccctgtgc cacctccaca gtagtaatga
     3661 actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
     3721 gagttgcgcg acgccgagcc ggttct
//
