LOCUS       sequence-277279-        1265 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1265)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0810
FEATURES             Location/Qualifiers
     source          1..1265
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcgtcccg gtgctccgag ccctacccgg aggtctccag aatccctacc
       61 gtcaggggat gcaacggctc cctgtctggt gccctctcct gctgcgagga ctcggcccgg
      121 ggctcgggcc cgcccaaggc ccctacggtg gccgagggtc ccagctcctg ccttcggcgg
      181 aacgtgatca gcgagaggga gcgcaggaag cggatgtcgt tgagctgtga gcgtctgcgg
      241 gccctgctgc cccagttcga tggccggcgg gaggacatgg cctcggtcct ggagatgtct
      301 gtgcagttcc tgcggcttgc cagcgccctg gggcccagtc aggagcagca cgctattctt
      361 gcttcctcca aggaaatgtg gcactcgttg caggaggatg ttttacagtt gacgttgtcg
      421 agtcagattc aagcaggtgt gccagaccct gggacgggag cgtccagcgg gactcgaacc
      481 ccagatgtga aggcgtttct ggaaagtcct tggtccctgg atccagcgtc ggccagccca
      541 gagcccgtgc cgcacatcct tgcgtcctcc aggcagtggg accccgcgag ctgcacgtcc
      601 ctgggcacgg acaagtgtga ggcactgttg gggctgtgcc aggtgcgggg tgggctgccc
      661 cctttctcag aaccttccag cctggtgccg tggcccccag gccggagtct tcctaaggct
      721 gtgaggccac ccctgtcctg gcctccgttc tcgcagcagc agaccttgcc cgtgatgagc
      781 ggggaggccc ttggctggct gggccaggct gggcccctgg ccatgggggc tgcacctctg
      841 ggggagccag ccaaggagga ccccatgctg gcgcaggagg ccgggtctgc gttggggtct
      901 gatgtggacg atgggacgtc cttcctgctg actgctggtc ccagctcgtg gccggggtct
      961 ctggagggtc gaggaggcag tggccctgca tgggccccag ctgagagcag tccactggat
     1021 gttggagagc caggcttcct aggggaccct gagcttggct cccaggagct ccaggacagc
     1081 cctctggagc cgtggggcct ggatgtggac tgtgcaggcc tggccctgaa ggacgaggtg
     1141 gagagcatct tccctgactt ctttgcctgc tagtaatgaa ctagtccgta acttgaaagt
     1201 atttcgattt cttggcttta tatatcttgt ggaaaggacg atcgcagtag gatccctcgg
     1261 caagg
//
