LOCUS       sequence-277282-        1466 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1466)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0811
FEATURES             Location/Qualifiers
     source          1..1466
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagttggc gactcgctac cagatcccta aagaagtggc tgacatcttt
       61 aacgccccca gtgatgatga agagtttgtt ggcttccgag atgatgttcc catggaaacc
      121 ctctcgtcag aggagagctg cgatagtttt gactcactag agtcagggaa acagcaggat
      181 gtgcgctttc attccaaata cttcacagaa gagctaagaa gaatttttat agaggacact
      241 gactcagaga ctgaggattt tgcaggattt acgcagagtg atctgaatgg aaagactaac
      301 ccagaagtaa tggtcgtgga gtcagatttg agtgatgatg gcaaagcatc tttggtgagc
      361 gaggaagagg aagatgaaga agaagataag gctaccccta gaagaagcag gtctagaaga
      421 agtagtattg gtcttcgagt agcctttcag ttccccacca agaagctggc caacaaacca
      481 gataaaaaca gttcttccga gcagttgttt tctagcgcac gcttacagaa tgagaaaaaa
      541 acaattcttg aaagaaagaa agactgtaga caggtgatac aaagggaaga ttctacctct
      601 gagtctgagg atgactctcg ggatgagagc caggagagtt cagatgcttt gctgaaaagg
      661 accatgaaca tcaaggagaa caaagccatg cttgcccagt tattggcgga attgaactcg
      721 atgccagatt tcttcccagt acgaacccca acctcagctt ctaggaagaa gacagtgagg
      781 cgggccttct cggagggaca gatcacgcgg cgtatgaacc caacccggag tgcgcggcct
      841 cctgagaagt ttgctctaga gaacttcact gtctcagccg ctaaatttgc ggaagagttt
      901 tacagcttcc gaagaaggaa gacaattggg gggaaatgcc gggagtacag acgacgtcac
      961 cgtatatctt cttttcggcc agtggaggat atcaccgaag aggacttaga aaatgttgcc
     1021 ataactgttc gagataaaat ctatgataaa gttctgggta acacgtgcca tcagtgtcga
     1081 caaaagacca tcgacaccaa gacagtgtgt cggaaccagg gttgctgtgg tgtgcgagga
     1141 cagttctgtg gaccatgcct gcggaaccgc tatggggagg atgtcagatc ggcattgctg
     1201 gacccggatt gggtgtgtcc cccctgtcgt gggatctgca attgcagcta ctgtcggaag
     1261 cgtgacggcc gctgtgccac aggaatcctc attcatctgg ccaagtttta tggttatgac
     1321 aatgttaagg aatatctgga gagcttacaa aaggagctgg tagaagacaa ttagtaatga
     1381 actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
     1441 gacgcctact tccacagtcg gaatcc
//
